Description

Sulpho-Nhs-Ss-Biotin is a specialized, heterobifunctional crosslinking reagent designed for advanced bioconjugation applications. This compound is critical for researchers and manufacturers who require precise, stable, and site-specific labeling of biomolecules, particularly in aqueous environments. It is an essential tool for professionals in biopharmaceutical development, diagnostic assay manufacturing, and proteomics research, enabling the creation of highly sensitive and specific detection systems.

Application

• Antibody-Drug Conjugate (ADC) Development: For the site-specific conjugation of cytotoxic payloads to monoclonal antibodies via amine groups.
• Diagnostic Kit Manufacturing: Used to label antibodies, antigens, or oligonucleotides with enzymes, fluorophores, or other reporter molecules for ELISA, lateral flow, and other immunoassays.
• Protein and Peptide Labeling: Enables the covalent attachment of biotin tags to proteins for purification or detection using streptavidin-based systems.
• Surface Functionalization: For immobilizing biomolecules onto biosensor chips, microarrays, and other solid supports to create bioactive surfaces.
• Cell Surface Engineering: Facilitates the tagging of cell surface proteins with probes for flow cytometry, microscopy, and tracking studies.
• Next-Generation Bioconjugation: Serves as a key reagent in the development of novel therapeutic and diagnostic platforms requiring controlled linkage chemistry.

Basic Information

Product Name	Sulpho-Nhs-Ss-Biotin
CAS No.	858128-64-0
Molecular Formula	C19H30N4O9S3
Molecular Weight	554.66 g/mol
Synonyms	Sulfo-NHS-SS-Biotin; Sulfosuccinimidyl 2-(biotinamido)ethyl-1,3'-dithiopropionate; N-(3-[2-(Biotinamido)ethyldithio]propionyloxy)sulfosuccinimide sodium salt; Sulfo-NHS-LC-Biotin (SS); EZ-Link Sulfo-NHS-SS-Biotin; Biotinylation reagent, water-soluble, cleavable; Sulfo-NHS-SS-Biotin sodium salt; 1,3-Dithio-2-(biotinylamido)propyl sulfosuccinimide ester
EINECS	Contact for details

Quality Control

Every batch of Sulpho-Nhs-Ss-Biotin is manufactured and tested under a strict quality management system. We ensure compliance with high-purity standards suitable for research and in-vitro diagnostic use. Comprehensive analytical testing, including HPLC for purity and identity confirmation, is performed. A detailed Certificate of Analysis (COA) is provided with each shipment, guaranteeing traceability and consistent performance for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO
Functional Activity	Conforms (by biotinylation assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.