Description

Insulin, N(Alpha)(B1), Biotinyl-ε-Aminocaproyl- is a chemically modified insulin derivative designed for advanced biochemical research and diagnostic applications. This biotinylated analog is critical for studies involving protein-protein interactions, receptor binding assays, and signal transduction pathways due to its high-affinity binding to streptavidin. It is primarily utilized by researchers and developers in the pharmaceutical, biotechnology, and life science sectors for creating sensitive detection systems and targeted therapeutic probes.

Application

• Biochemical Research: A key reagent for studying insulin receptor interactions, internalization, and downstream signaling mechanisms.
• Diagnostic Assay Development: Used in the development of ELISA, Western blot, and other immunoassays for the detection and quantification of insulin-related activity.
• Affinity Purification: Serves as a ligand for streptavidin-based purification columns to isolate insulin-binding proteins or complexes.
• Cell Biology Studies: Employed in fluorescence microscopy and flow cytometry for visualizing insulin receptor localization and trafficking on cell surfaces.
• Drug Discovery: Used in high-throughput screening (HTS) platforms to identify novel modulators of the insulin signaling pathway.
• Proteomics: Acts as a bait protein in pull-down experiments to map the insulin interactome.

Basic Information

Product Name	Insulin, N(Alpha)(B1), Biotinyl-ε-Aminocaproyl-
CAS No.	89958-37-2
Molecular Formula	C267H402N70O78S6 (Bovine Insulin derivative)
Molecular Weight	~5850 g/mol (Contact for exact value)
Synonyms	Biotinylated Insulin; Biotin-ε-Aminocaproyl-Insulin; Nα(B1)-Biotinyl-6-Aminocaproyl Insulin; Biotin-Insulin Conjugate; Insulin-Biotin; Biotin Labeled Insulin; Biotinamidocaproyl Insulin; Insulin (Biotinylated)
EINECS	Contact for details

Quality Control

Our Insulin, N(Alpha)(B1), Biotinyl-ε-Aminocaproyl- is produced under stringent quality management protocols. Each batch is characterized using advanced analytical techniques including HPLC, mass spectrometry, and bioactivity assays to ensure high purity, correct molecular structure, and preserved biological function. Certificates of Analysis (COA) detailing purity, biotin incorporation ratio, and endotoxin levels are provided with every shipment to support your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to keep the lyophilized powder desiccated. Avoid repeated freeze-thaw cycles of prepared solutions.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Biotin Incorporation (Molar Ratio)	~1:1 (Insulin:Biotin)
Biological Activity	Retains insulin receptor binding activity
Water Content (KF)	≤ 5.0%
Endotoxin	< 10 EU/mg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.