Description

Bz-Phe-Val-Arg-Amc CAS NO 88899-22-3 is a fluorogenic peptide substrate designed for the sensitive detection and quantification of specific protease activity. This compound is critical for biochemical research and diagnostic assay development, providing a reliable tool for enzyme kinetics and inhibitor screening. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and life sciences industries for high-throughput screening and mechanistic studies.

Application

• Protease Activity Assays: As a selective substrate for measuring the enzymatic activity of specific serine or cysteine proteases in vitro.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize novel protease inhibitors for therapeutic development.
• Biochemical Research: A fundamental tool for studying enzyme kinetics, catalytic mechanisms, and substrate specificity in academic and industrial laboratories.
• Diagnostic Kit Development: Employed in the formulation of fluorogenic assays for clinical diagnostics and biomarker detection.
• Quality Control in Biologics: Monitoring protease activity during the production and purification of protein-based pharmaceuticals.

Basic Information

Product Name	Bz-Phe-Val-Arg-Amc
CAS No.	88899-22-3
Molecular Formula	C34H48N8O6
Molecular Weight	664.80 g/mol
Synonyms	Benzoyl-Phe-Val-Arg-7-amido-4-methylcoumarin; Bz-Phe-Val-Arg-AMC; Boc-Phe-Val-Arg-AMC (common abbreviation); Benzoyl-L-phenylalanyl-L-valyl-L-arginine 7-amido-4-methylcoumarin; Protease Substrate VII; Fluorogenic Thrombin Substrate; AMC-based peptide substrate; 7-Amido-4-methylcoumarin conjugate of Bz-Phe-Val-Arg
EINECS	Contact for details

Quality Control

Our Bz-Phe-Val-Arg-Amc is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and spectroscopic identification (NMR, MS), to ensure it meets the high standards required for research and assay development. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.