Description

Vimentin From Bovine Lens is a high-purity, native intermediate filament protein isolated from bovine ocular tissue. This product is essential for biochemical and biomedical research, serving as a critical standard and substrate in studies of cytoskeletal structure, cell signaling, and disease pathology. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in advanced cellular and molecular biology.

Application

• Biochemical Research Standard: Used as a purified protein standard in Western blotting, ELISA, and immunocytochemistry for vimentin detection and quantification.
• Antigen Production: Serves as a high-quality immunogen for generating specific polyclonal and monoclonal antibodies.
• Cell Biology Studies: Employed in in vitro assays to investigate intermediate filament assembly, dynamics, and interactions with other cellular components.
• Disease Mechanism Research: Applied in studies of epithelial-to-mesenchymal transition (EMT), fibrosis, cancer metastasis, and cataract formation.
• Diagnostic Development: Used as a coating antigen or control in the development of immunoassays and diagnostic kits for autoimmune diseases and certain cancers.
• Pharmaceutical Screening: Acts as a target protein in high-throughput screening platforms for drug discovery programs focused on cytoskeleton-related disorders.

Basic Information

Product Name	Vimentin From Bovine Lens
CAS No.	88566-40-9
Molecular Formula	Contact for details
Molecular Weight	~57 kDa (monomer)
Synonyms	Vimentin
Bovine Lens Vimentin
VIM
Intermediate Filament Protein, Type III
Native Vimentin
Vimentin (Bovine)
Vimentin, Lens
EINECS	Contact for details

Quality Control

Our Vimentin From Bovine Lens is produced and handled under controlled conditions to ensure batch-to-batch consistency and biological activity. Each lot is subjected to rigorous purity analysis, including SDS-PAGE and immunoassay, to confirm identity and minimize contaminants. A comprehensive Certificate of Analysis (COA) detailing purity, concentration, and biological activity is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Reconstituted solutions should be aliquoted and stored at -70°C to -80°C to prevent repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); allow vials to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (SDS-PAGE)	Single band at ~57 kDa
Identification (Immunoblot)	Positive reactivity with anti-vimentin antibodies
Purity (by SDS-PAGE)	≥ 90%
Protein Content (Biuret)	≥ 95% of dry weight
Biological Activity	Capable of filament assembly in vitro
Endotoxin Level (LAL)	< 1.0 EU/mg
Solubility	Soluble in neutral aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.