Description

2H-Tetrazolium, 3-(4-Chlorophenyl)-5-(1H-Indol-3-Yl)-2-Phenyl-, Iodide is a specialized tetrazolium salt derivative that serves as a critical reagent in biochemical and analytical research. Its core value lies in its role as a redox indicator and chromogenic substrate, enabling sensitive detection and quantification in enzymatic assays. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors, where precise measurement of metabolic activity or enzyme function is required.

Application

• As a chromogenic substrate in cell viability and cytotoxicity assays (e.g., MTT assay alternatives).
• Used as a redox indicator in biochemical research for studying dehydrogenase enzyme activity.
• Critical reagent in high-throughput screening (HTS) platforms for drug discovery and development.
• Employed in microbiology for bacterial cell proliferation and metabolic activity studies.
• Component in diagnostic kit formulations for clinical and research laboratory use.
• Utilized in material science research involving redox-active polymers or coatings.

Basic Information

Product Name	2H-Tetrazolium, 3-(4-Chlorophenyl)-5-(1H-Indol-3-Yl)-2-Phenyl-, Iodide
CAS No.	87582-49-8
Molecular Formula	C21H15ClIN5
Molecular Weight	515.73 g/mol
Synonyms	3-(4-Chlorophenyl)-5-(1H-indol-3-yl)-2-phenyl-2H-tetrazolium iodide; 4-Chloro-3-(1H-indol-3-yl)-2,5-diphenyl-2H-tetrazolium iodide; Tetrazolium Violet Iodide; TV-I; Iodonitrotetrazolium Violet; INT Violet; (4-Chlorophenyl)-3-(1H-indol-3-yl)-2,5-diphenyl-2H-tetrazolium iodide
EINECS	Contact for details

Quality Control

Our 2H-Tetrazolium, 3-(4-Chlorophenyl)-5-(1H-Indol-3-Yl)-2-Phenyl-, Iodide is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and spectroscopic identification. A Certificate of Analysis (COA) detailing all test results is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to prevent degradation. Keep the container tightly sealed when not in use to minimize exposure to atmospheric moisture.

Specification

Item	Specification
Appearance	Dark purple to violet crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.5%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.