Description

β-(n-(7-Nitro-2,1,3-Benzoxadiazol-4-Yl))Alanyl-Coenzyme A is a specialized, fluorescently labeled Coenzyme A derivative, designed for advanced biochemical research and diagnostic applications. This compound serves as a critical tool for the sensitive detection and quantification of enzymatic activity, particularly in acyltransferase and other CoA-dependent assays. It is essential for researchers and developers in pharmaceutical discovery, proteomics, and clinical diagnostics who require high-purity, reliable reagents for tracing metabolic pathways and developing novel assays.

Application

• Enzymatic Activity Assays: As a fluorescent substrate for real-time monitoring of acyl-CoA synthetases, acyltransferases, and other CoA-utilizing enzymes.
• Biomarker Detection & Proteomics: Used in the labeling and detection of proteins and peptides in complex biological samples via fluorescence-based techniques.
• Drug Discovery & High-Throughput Screening (HTS): Enables the development of sensitive fluorescence assays to identify and characterize enzyme inhibitors or activators.
• Metabolic Pathway Analysis: Tracer for studying lipid metabolism, fatty acid activation, and other CoA-mediated biochemical processes.
• Diagnostic Kit Development: A key component in the formulation of research-use-only (RUO) and in-vitro diagnostic (IVD) kits for clinical analysis.
• Academic & Industrial Research: Fundamental reagent for mechanistic studies in biochemistry and molecular biology laboratories.

Basic Information

Product Name	β-(n-(7-Nitro-2,1,3-Benzoxadiazol-4-Yl))Alanyl-Coenzyme A
CAS No.	86848-57-9
Molecular Formula	C34H48N10O22P3S
Molecular Weight	1058.80 g/mol
Synonyms	NBD-Ala-CoA; 7-Nitrobenz-2-oxa-1,3-diazol-4-yl-alanyl-coenzyme A; NBD-Alanyl-CoA; Coenzyme A, S-[2-[[[5-[[5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydro-2-furanyl]methoxyphosphinyl]oxy]-4-hydroxy-2-(phosphonooxy)-3-[[(2R)-2-(7-nitro-4-benzofurazanyl)amino]-1-oxo-3-(phenylmethoxy)propyl]amino]-1-oxobutyl]amino]ethyl] ester; Fluorescent CoA derivative; NBD-labeled Coenzyme A
EINECS	Contact for details

Quality Control

Our β-(n-(7-Nitro-2,1,3-Benzoxadiazol-4-Yl))Alanyl-Coenzyme A is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and spectroscopic methods for identity confirmation. Certificates of Analysis (COA) detailing purity, identity, and specific performance data are available upon request to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light will lead to degradation. Allow the vial to equilibrate to room temperature before opening to minimize condensation.

Specification

Item	Specification
Appearance	Yellow to orange lyophilized powder
Identification (HPLC/UV)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Solubility	Soluble in DMSO, Methanol, Water
Extinction Coefficient	~25,000 M-1cm-1 at ~465 nm
Water Content (KF)	< 5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.