Description

C6-Nbd-Ceramide CAS NO 86701-10-2 is a fluorescently labeled lipid derivative, specifically a ceramide analog tagged with the nitrobenzoxadiazole (NBD) fluorophore on a C6 acyl chain. This compound is a critical research tool for the real-time visualization and tracking of lipid metabolism, membrane trafficking, and intracellular signaling pathways. It is primarily utilized by researchers and scientists in the fields of cell biology, biochemistry, and pharmacology for advanced fluorescence microscopy and flow cytometry studies.

Application

• Fluorescent Labeling of Ceramide: Used as a vital probe to study the synthesis, transport, and metabolism of ceramides within live cells.
• Sphingolipid Research: Enables the investigation of sphingolipid-rich membrane domains (lipid rafts), cell signaling, and apoptosis mechanisms.
• Intracellular Trafficking Studies: Tracks the movement of lipids from the Golgi apparatus to the plasma membrane and other organelles.
• Membrane Biophysics: Serves as a tool to examine membrane fluidity, fusion, and organization using fluorescence techniques.
• Drug Discovery & Toxicology: Assists in screening compounds that affect sphingolipid pathways, relevant to cancer, neurodegenerative, and metabolic diseases.
• Diagnostic Assay Development: Can be incorporated into in vitro assays to detect enzymatic activity related to sphingolipid metabolism.

Basic Information

Product Name	C6-Nbd-Ceramide
CAS No.	86701-10-2
Molecular Formula	C31H48N4O6
Molecular Weight	572.74 g/mol
Synonyms	NBD C6-Ceramide; N-[6-[(7-Nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-D-erythro-sphingosine; C6-NBD-Cer; D-erythro-NBD C6-Ceramide; NBD-C6-Cer; 6-((N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoyl)sphingosine; Fluorescent Ceramide C6; Ceramide NBD C6
EINECS	Contact for details

Quality Control

Our C6-Nbd-Ceramide is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and fluorescence properties is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation and degradation. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Solubility	Soluble in DMSO, ethanol, DMF
Extinction Coefficient	~21,000 cm-1M-1 at ~465 nm
Fluorescence Emission	~534 nm (in methanol)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.