Description

Indo 1 Pentapotassium Salt is a high-purity, water-soluble salt derivative of the fluorescent calcium indicator Indo-1. This compound is essential for researchers and industrial developers requiring a stable, cell-permeant form for precise intracellular calcium ([Ca²⁺]i) quantification. It is a critical reagent for fluorescence microscopy, flow cytometry, and high-throughput screening applications. Key industries that rely on this product include biotechnology, pharmaceutical R&D, and advanced materials science.

Application

• Intracellular Calcium Imaging: Primary use as a rationetric fluorescent probe for measuring dynamic changes in cytosolic calcium concentrations in live cells.
• Flow Cytometry: Enables the analysis of calcium flux in cell populations, crucial for immunology and cell signaling studies.
• Drug Discovery & High-Throughput Screening (HTS): Used in assays to identify compounds that modulate calcium channels or calcium-dependent pathways.
• Neuroscience Research: Monitoring neuronal activity and synaptic transmission via calcium signaling events.
• Cardiovascular Research: Studying calcium handling in cardiomyocytes and smooth muscle cells.
• Loaded Indicator Preparation: Used with acetoxymethyl (AM) ester derivatives to create cell-permeant loading solutions for non-invasive cellular studies.

Basic Information

Product Name	Indo 1 Pentapotassium Salt
CAS No.	132319-56-3
Molecular Formula	C47H49K5N3O12
Molecular Weight	Contact for details
Synonyms	Indo-1, Pentapotassium Salt; Indo-1, K5 Salt; 1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N',N'-tetraacetic Acid Pentapotassium Salt; 5,5'-[1,2-Ethanediylbis[(2-methyl-5-oxido-1H-benzimidazole-4,6-diyl)imino(1-hydroxy-2-oxo-1,2-ethanediyl)]]bis[2-(2,7-dichloro-6-oxido-3-oxo-9H-xanthen-9-yl)]benzoic Acid Pentapotassium Salt; Calcium Indicator Indo-1, Pentapotassium Salt; Indo-1, cell impermeant salt
EINECS	Contact for details

Quality Control

Our Indo 1 Pentapotassium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and performance reliability for sensitive research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity, spectrophotometric analysis to confirm spectral properties, and functional validation in cell-based assays. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere or vacuum desiccation. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Fluorescence Properties	Excitation/Emission maxima conform to standard
Solubility	Freely soluble in aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.