Description

Bouin's Fluid (Histopathology Fixative) is a specialized chemical solution formulated for the critical preservation of biological tissue samples. Its primary value lies in providing superior cellular and structural fixation, which is essential for accurate histological diagnosis and research. This product is indispensable for pathology laboratories, biomedical research institutions, and pharmaceutical development facilities requiring high-quality tissue preparation for microscopic analysis. The CAS number for this compound is 1302-86-2.

Application

Bouin's Fluid is a gold-standard fixative in histotechnology, prized for its ability to preserve fine cellular detail and enhance staining contrast. Its primary and related applications include:

• Routine Histopathology: Standard fixation of biopsy and surgical specimens for diagnostic evaluation.
• Embryology and Small Tissue Studies: Excellent for fixing delicate tissues, embryos, and invertebrates due to its gentle yet penetrating action.
• Trichrome Staining Enhancement: Particularly effective as a pre-fixative for connective tissue stains like Masson's trichrome, improving color differentiation.
• Research & Development: Used in biomedical research for preserving tissue architecture in experimental animal models.
• Museum Specimen Preparation: Long-term preservation of anatomical specimens for educational collections.
• Veterinary Pathology: Tissue fixation for diagnostic and research purposes in animal health.

Basic Information

Product Name	Bouin's Fluid (Histopathology Fixative)
CAS No.	1302-86-2
Molecular Formula	Mixture (Picric Acid, Formaldehyde, Acetic Acid)
Molecular Weight	N/A (Composite Solution)
Synonyms	Bouin's Solution; Bouin's Fixative; Bouin Fluid; Picric Acid-Formalin-Acetic Acid Fixative; PFA Fixative (Bouin's Type); Histological Bouin's; Bouin's Aqueous; Alcohol Bouin's Fixative; Modified Bouin's Fluid
EINECS	Contact for details

Quality Control

Our Bouin's Fluid is manufactured under controlled conditions to ensure batch-to-batch consistency critical for reproducible histological results. Each lot is tested to verify the concentration and compatibility of its key components—picric acid, formaldehyde, and acetic acid. While not a single-compound pharmaceutical, production adheres to Good Manufacturing Practice (GMP) principles for laboratory reagents. A detailed Certificate of Analysis (COA) specifying component ratios, pH, and appearance is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, well-ventilated area at a controlled room temperature (15-25°C). Due to the presence of highly volatile and flammable components (formaldehyde, acetic acid), keep away from heat, sparks, and open flames. The picric acid component is a desensitized wet mass but requires handling away from strong alkalis and reducing agents. Keep container tightly sealed when not in use to prevent evaporation and absorption of moisture.

Specification

Item	Specification
Appearance	Clear, yellow solution
Identification (Components)	Complies (Picric Acid, Formaldehyde, Acetic Acid)
Composition (Typical)	Saturated picric acid aqueous solution, formaldehyde, glacial acetic acid (75:25:5 ratio)
pH	Approximately 1.0 - 2.0
Foreign Particulate Matter	Essentially free
Performance Test (Histological Fixation)	Complies (Preserves nuclear and connective tissue detail)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.