Description

Nnk-D3, 4-(Methyl-D3-Nitrosoamino)-1-(3-Pyridinyl)-1-Butanone is a deuterium-labeled isotopologue of the tobacco-specific nitrosamine NNK (4-(Methylnitrosamino)-1-(3-pyridinyl)-1-butanone). This compound matters as a critical internal standard and reference material for precise analytical quantification, enabling researchers to correct for losses during sample preparation and analysis. It is essential for laboratories in the fields of toxicology, analytical chemistry, and regulatory science, particularly those focused on the accurate detection and measurement of carcinogenic nitrosamines in complex matrices like tobacco, food, and environmental samples.

Application

• Internal Standard for Mass Spectrometry (MS): Serves as a deuterated internal standard in GC-MS or LC-MS/MS methods for the quantification of NNK, improving accuracy and precision in analytical results.
• Tobacco and Smoke Research: Used in studies to measure the levels of tobacco-specific nitrosamines (TSNAs) in tobacco products, smoke, and related materials.
• Food Safety Analysis: Applied in methods to detect and quantify nitrosamine contaminants in food products, such as cured meats and certain beverages.
• Pharmacokinetic and Metabolic Studies: Employed as a tracer to study the absorption, distribution, metabolism, and excretion (ADME) of NNK in biological systems.
• Environmental Monitoring: Facilitates the analysis of nitrosamine levels in environmental samples, including water and air.
• Method Development and Validation: A key component in developing, optimizing, and validating new analytical protocols for nitrosamine detection to meet regulatory requirements.
• Quality Control and Assurance: Used in laboratory quality control programs to ensure the reliability and consistency of nitrosamine testing.
• Regulatory Compliance Testing: Supports testing for compliance with international health and safety regulations concerning nitrosamine limits.

Basic Information

Item	Detail
Product Name	Nnk-D3, 4-(Methyl-D3-Nitrosoamino)-1-(3-Pyridinyl)-1-Butanone
CAS No.	86270-92-0
Molecular Formula	C10H10D3N3O2
Molecular Weight	210.24 g/mol
Synonyms	NNK-d3; 4-(N-Methyl-D3-nitrosamino)-1-(3-pyridinyl)-1-butanone; NNK (methyl-d3); 4-[(Methyl-d3)nitrosamino]-1-(3-pyridinyl)-1-butanone; Deuterated NNK; NNK Internal Standard; 4-(Methyl-d3-nitrosoamino)-1-(3-pyridyl)-1-butanone; CAS 86270-92-0
EINECS	Contact for details

Quality Control

Our deuterated standards, including Nnk-D3, are produced under strict quality protocols to ensure high chemical purity and isotopic enrichment, which are critical for reliable analytical performance. Each batch is rigorously tested using advanced techniques such as NMR and mass spectrometry to confirm structural identity, isotopic purity (typically >98 atom % D), and chemical purity. A comprehensive Certificate of Analysis (COA) is provided, detailing assay results, purity, and batch-specific data to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. For short-term use, storage at 2-8°C is acceptable. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or solution (as specified)
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98%
Isotopic Purity	≥ 98 atom % D
Chemical Purity	≥ 98%
Solvent (if supplied as solution)	Methanol, Acetonitrile, or as specified
Concentration (if supplied as solution)	100 µg/mL, 1.0 mg/mL, or as specified

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.