Description

Tetramethylrhodaminylphalloidine is a high-purity, fluorescently labeled derivative of the potent toxin phalloidin, specifically designed for advanced microscopy applications. This compound is critical for researchers requiring precise, high-contrast visualization of filamentous actin (F-actin) structures within fixed cells and tissues. It is an essential tool for life science research, diagnostic development, and cellular biology studies in academic, pharmaceutical, and biotechnology sectors. The product is supplied under stringent quality control to ensure optimal performance and batch-to-batch consistency for reliable experimental results.

Application

• Fluorescence Microscopy: Primary application for labeling and visualizing F-actin in fixed cell preparations using fluorescence microscopes.
• Confocal and Super-Resolution Imaging: Enables detailed 3D reconstruction and high-resolution analysis of the cytoskeleton architecture.
• Cytoskeleton Research: Used to study actin dynamics, cell morphology, motility, and the effects of drugs or genetic modifications on the actin network.
• Neuroscience Studies: Labels actin filaments in neurons to investigate growth cones, synapses, and structural plasticity.
• Pathology and Diagnostics: Acts as a staining reagent in research focused on diseases involving cytoskeletal abnormalities, such as certain cancers or neurological disorders.
• High-Content Screening (HCS): Employed in automated imaging platforms for drug discovery campaigns targeting the cytoskeleton.

Basic Information

Product Name	Tetramethylrhodaminylphalloidine
CAS No.	86107-52-0
Molecular Formula	C₆₀H₇₈N₈O₁₃S⁺ (typically as a salt, e.g., with methanesulfonate)
Molecular Weight	~1111.4 g/mol (free base)
Synonyms	TRITC-Phalloidin; Tetramethylrhodamine isothiocyanate phalloidin; Rhodamine-phalloidin; Phalloidin–tetramethylrhodamine conjugate; Phalloidin-TRITC; (3R,4S,7S,10R,13S,16S,19R,22S,25S,28S,31S,34S,37S)-34-[(2S)-2-[[(6S)-6-[[(6S)-6-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-Amino-3-hydroxypropanoyl]amino]-3-hydroxypropanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-3-methylbutanoyl]amino]-3-hydroxypropanoyl]amino]-3-(1H-indol-3-yl)propanoyl]amino]-3-methylbutanoyl]amino]-3-hydroxypropanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-3-methylbutanoyl]amino]-3-hydroxypropanoyl]amino]-3-(1H-indol-3-yl)propanoyl]amino]-3-methylbutanoyl]amino]-3-hydroxypropanoyl]amino]-22-(2-amino-2-oxoethyl)-37-(carboxymethyl)-10,13,16,19,25,28,31-heptamethyl-3,4,7,22,25,28,31,34,37-nonaoxo-1,2,3,4,7,8,9,10,13,14,15,16,19,20,21,22,25,26,27,28,31,32,33,34,37,38-sixacosahydrocyclopenta[a]pyrrolo[2,1-c][1,4,7,10,13,16,19,22,25,28,31,34]dodecaazacyclohexatriacontin-6-yl 5-(3-carboxy-2,6-dimethyl-4-(2,4,6-trimethylphenyl)-1,4-dihydropyridin-5-yl)-2,6-dimethyl-4-(2,4,6-trimethylphenyl)-1,4-dihydropyridine-3-carboxylate (IUPAC)
EINECS	Contact for details

Quality Control

Every batch of Tetramethylrhodaminylphalloidine is manufactured and tested to meet the exacting standards required for life science research. Our quality assurance includes rigorous analytical testing to ensure high purity, correct labeling, and optimal fluorescence performance. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specifications such as purity (HPLC), identity (MS, NMR), fluorescence properties, and functional performance in actin staining assays.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. The product is hygroscopic (moisture-sensitive) and light-sensitive; allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation. For long-term stability, store desiccated under inert atmosphere.

Specification

Item	Specification
Appearance	Red to purple solid or film
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Area %)
Fluorescence Emission	~ 575 nm (bound to F-actin)
Extinction Coefficient (ε)	~95,000 cm⁻¹M⁻¹ at 540 nm
Solubility	Soluble in methanol, DMSO
Functional Staining	Tested in fixed cell assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.