Description

Fibronectin is a high-molecular-weight glycoprotein that plays a critical role in cell adhesion, migration, growth, and differentiation. Its primary commercial value lies in its ability to create a biologically active substrate for cell culture, significantly improving cell attachment and proliferation in vitro. This product is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and biomedical engineering sectors, particularly for applications in tissue engineering, stem cell research, and advanced therapeutic development.

Application

• Cell Culture Substrate Coating: Used to coat tissue culture plasticware, glass, or biomaterials to enhance the attachment and spreading of anchorage-dependent cells, including primary cells and stem cells.
• Tissue Engineering and Regenerative Medicine: Incorporated into scaffolds and hydrogels to promote cell infiltration, tissue integration, and wound healing.
• Cancer and Cell Biology Research: Employed in studies investigating cell-matrix interactions, metastasis, and signaling pathways related to adhesion and migration.
• Diagnostic Assay Development: Serves as a capture protein or a component in assays designed to study integrin function and cell adhesion molecules.
• Biomaterial Functionalization: Applied to modify the surface of medical implants and devices to improve biocompatibility and host tissue integration.
• Virus Propagation: Used in certain viral production systems where cell attachment is a critical factor for efficient virus yield.

Basic Information

Product Name	Fibronectin
CAS No.	86088-83-7
Molecular Formula	Contact for details
Molecular Weight	~440 kDa (dimer)
Synonyms	Cold-Insoluble Globulin (CIG); LETS Protein (Large External Transformation-Sensitive protein); Cell-Surface Protein; Fibronectin Human; Plasma Fibronectin; Cellular Fibronectin; FN; Uronic Acid-Rich Protein; Antigen p245
EINECS	Contact for details

Quality Control

Our Fibronectin is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and biological activity. Each lot is characterized for purity, sterility, and functional performance in cell-based assays. A comprehensive Certificate of Analysis (COA) detailing identity, purity, endotoxin level, and biological activity is provided with every shipment to support your regulatory and research requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Reconstituted solutions should be aliquoted and stored at -20°C to -80°C to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive).

Specification

Item	Specification
Appearance	White or off-white lyophilized powder or frozen solution
Identification (SDS-PAGE)	Consistent with Fibronectin standard
Purity (SDS-PAGE)	> 95%
Protein Content (UV Absorbance)	≥ 1 mg/mL (as specified per lot)
Endotoxin	< 1.0 EU/mg
Biological Activity (Cell Adhesion)	Promotes > 80% cell attachment at recommended coating concentration
Sterility (where applicable)	Sterile, 0.22 µm filtered

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.