Description

Pyr-Phe-Leu-Pna is a synthetic tripeptide-derived chromogenic substrate widely used in enzymatic assays for protease activity detection and inhibitor screening. It delivers high sensitivity and reproducibility in quantitative biochemical analysis, enabling reliable kinetic profiling of serine and cysteine proteases. This compound is essential for research and development laboratories in pharmaceutical discovery, diagnostic reagent manufacturing, and academic life sciences. Pyr-Phe-Leu-Pna CAS NO 85901-57-1 is supplied under strict quality control to ensure batch-to-batch consistency and assay fidelity.

Application

• Enzymatic assay substrate for chymotrypsin-like proteases (e.g., cathepsins, elastase, thrombin)
• High-throughput screening (HTS) of protease inhibitors in drug discovery pipelines
• Quality control testing of proteolytic enzyme preparations in biopharmaceutical production
• Development and validation of clinical diagnostic kits for inflammatory and degenerative disease biomarkers
• Research tool in apoptosis and protein turnover studies involving caspase- and calpain-family enzymes
• Calibration standard in spectrophotometric and fluorometric assay optimization

Basic Information

Product Name	Pyr-Phe-Leu-Pna
CAS No.	85901-57-1
Molecular Formula	C30H36N6O7
Molecular Weight	608.65 g/mol
Synonyms	Pyroglutamyl-Phenylalanyl-Leucyl-p-nitroanilide; Pyr-Phe-Leu-pNA; Z-Phe-Leu-pNA (incorrect but commonly misused); PyroGlu-Phe-Leu-p-nitroanilide; PGLU-PHE-LEU-PNA; H-Pyr-Phe-Leu-pNA-OH; p-Nitroanilide derivative of Pyr-Phe-Leu; N-(4-Nitrophenyl)glycine tripeptide amide analog
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. All batches are verified for identity (IR, HPLC retention time), purity (≥98.5% by HPLC), and absence of critical impurities (e.g., residual solvents, heavy metals). Testing follows ACS Reagent Grade and EP/USP general chapters where applicable, supporting use in regulated R&D environments.

Storage

Preserve in a tightly closed container, protected from light. Store at 2–8 °C under inert atmosphere (argon or nitrogen) to prevent degradation. Due to its hygroscopic nature, minimize exposure to ambient humidity; allow container to equilibrate to room temperature before opening. Handle in a dry, well-ventilated area.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identity (IR)	Conforms to reference spectrum
Assay (HPLC)	≥98.5%
Related Substances (HPLC)	Single impurity ≤0.5%; Total impurities ≤1.5%
Residual Solvents (GC)	Meets ICH Q3C limits
Water Content (KF)	≤0.5%
Heavy Metals	≤10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.