Description

Hemoglobin Tokoname is a highly purified, biologically active hemoglobin preparation derived from bovine or equine sources using proprietary low-temperature fractionation and stabilization techniques. It serves as a critical reference standard and functional biomaterial for diagnostic assay development, oxygen-binding studies, and biomedical research applications requiring native tetrameric structure and physiological reactivity. This product is essential for manufacturers of blood gas analyzers, point-of-care hemoglobinometers, and in vitro diagnostic (IVD) kit developers seeking traceable, lot-to-lot consistent hemoglobin with verified spectral and functional integrity. Hemoglobin Tokoname CAS NO 85898-26-6 is supplied as a lyophilized powder under inert atmosphere to preserve conformational stability.

Application

• Primary reference standard for calibration of clinical hemoglobin analyzers and blood gas instruments
• Active component in in vitro diagnostic (IVD) kits for anemia screening and hemoglobinopathy detection
• Substrate in enzymatic and spectroscopic assays measuring heme peroxidase activity or oxidative stability
• Model protein for studying oxygen binding kinetics, allosteric regulation, and heme-protein interactions
• Stabilizing agent and oxygen carrier in cell culture media formulations for hypoxia-sensitive primary cells
• Raw material for conjugation-based biosensors and hemoglobin-based oxygen carriers (HBOCs) R&D
• Quality control benchmark in pharmacopeial testing (USP <1057>, EP 2.6.27) for hemoglobin-containing products
• Standard in regulatory submissions requiring well-characterized hemoglobin for biocompatibility and extractables testing

Basic Information

Product Name	Hemoglobin Tokoname
CAS No.	85898-26-6
Molecular Formula	C2952H4664N812O840S8Fe4 (approximate for human HbA; bovine variant differs slightly)
Molecular Weight	~64,500 g/mol (tetrameric form; monomeric subunit ~16,125 Da)
Synonyms	Hemoglobin (bovine), Hemoglobin (equine), Oxyhemoglobin, Methemoglobin (reduced form available), Globin-heme complex, HbTok, Tokoname Hemoglobin, Hemoglobin Standard Grade, Hemoglobin Reference Material, Hb-85898-26-6
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. Each batch is verified for identity (UV-Vis spectroscopy, SDS-PAGE), purity (HPLC-SEC), residual solvent content (GC), endotoxin levels (<0.5 EU/mg), and functional oxygen-binding capacity (CO-difference spectroscopy). Testing aligns with USP <1057> “Reference Standards” and ISO 17034:2016 requirements for certified reference materials. All lots are fully traceable to source material origin and manufacturing process parameters.

Storage

Preserve in a tightly closed container, protected from light. Store at 2–8°C under inert atmosphere (argon or nitrogen) to prevent oxidation and denaturation. Due to its hygroscopic nature, maintain relative humidity below 30% during storage and handling. Avoid repeated freeze-thaw cycles. Reconstituted solution should be used immediately or stored at 2–8°C for no longer than 24 hours.

Specification

Item	Specification
Appearance	Red to dark red lyophilized powder
Identification (UV-Vis)	λmax at 414 nm (Soret band), 541 nm and 577 nm (α/β bands); A414/A577 ratio ≥ 3.8
Assay (by UV absorbance)	95.0–102.0% (as hemoglobin, based on ε414 = 125,000 M−1cm−1)
Purity (HPLC-SEC)	≥ 98.5% tetrameric hemoglobin
Aggregates (HPLC-SEC)	≤ 1.0%
Fragmentation (HPLC-SEC)	≤ 0.5%
Endotoxin	< 0.5 EU/mg
Residual moisture (Karl Fischer)	1.0–3.5%
pH of 1% solution	6.8–7.2

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.