Description

Fentanyl-1-n-Oxide is a pharmacologically active metabolite and analytical reference standard derived from the potent synthetic opioid fentanyl. It serves as a critical tool for forensic toxicology, pharmaceutical R&D, and bioanalytical method validation—enabling precise quantification and structural confirmation in complex biological matrices. This compound is essential for laboratories engaged in controlled substance monitoring, metabolic pathway studies, and regulatory compliance testing across clinical, research, and law enforcement settings. Fentanyl-1-n-Oxide CAS NO 85893-37-4 is supplied under stringent quality controls to ensure batch-to-batch consistency and traceability.

Application

• Reference standard for LC-MS/MS and GC-MS quantification of fentanyl metabolites in human plasma, urine, and postmortem specimens
• Calibration material in forensic toxicology laboratories accredited to ISO/IEC 17025
• Metabolic stability assessment in human liver microsome (HLM) and recombinant CYP enzyme assays
• Method development and validation for anti-doping control programs (WADA-compliant workflows)
• Quality assurance in pharmaceutical manufacturing of fentanyl analogs and extended-release formulations
• Stability-indicating assay development for fentanyl oxidation pathways under ICH Q1–Q5 guidelines
• Proficiency testing material for national reference laboratories and CDC-certified drug testing programs
• Research-grade compound for structure–activity relationship (SAR) studies on opioid receptor binding affinity

Basic Information

Product Name	Fentanyl-1-n-Oxide
CAS No.	85893-37-4
Molecular Formula	C22H28N2O2
Molecular Weight	352.47 g/mol
Synonyms	Fentanyl N-oxide; Fentanyl-1-N-oxide; N-Oxofentanyl; Fentanyl oxide; Fentanyl N-oxide (USP); 1-(2-Phenylethyl)-4-(N-phenylpropionamido)piperidine N-oxide; N-(1-(2-Phenylethyl)-4-piperidinyl)-N-phenylpropanamide N-oxide; Fentanyl N-oxide (EP); Fentanyl N-oxide (JP); Fentanyl N-oxide (ISO); Fentanyl N-oxide (IUPAC: 1-[2-phenylethyl]-4-[N-phenylpropanamido]piperidine N-oxide)
EINECS	Not listed (not commercially registered under EINECS)

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request and include full chromatographic purity data (HPLC-UV), identity confirmation (IR and NMR), residual solvent profiling (GC), heavy metal limits (≤10 ppm), and water content (Karl Fischer). All batches meet or exceed USP Reference Standard criteria for identity, purity, and assay accuracy. Testing is performed in ISO/IEC 17025-accredited laboratories following ICH Q5, Q6, and Q7 guidelines.

Storage

Preserve in a tightly closed container, protected from light. Store at 2–8°C under inert atmosphere (argon or nitrogen) to prevent degradation. Due to its hygroscopic nature, maintain low-humidity conditions (<30% RH) and minimize exposure to ambient air during handling. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	98.5% – 101.0%
Related Substances (HPLC)	Each impurity ≤ 0.3%; Total impurities ≤ 1.0%
Residual Solvents (GC)	Meets ICH Q3C Class 3 limits
Water Content (KF)	≤ 0.5%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.