Description

3,4-Dinitrophenyl-β-Galactopyranoside is a specialized chromogenic and fluorogenic substrate for the enzyme β-galactosidase. This compound is critical for biochemical research and diagnostic applications, enabling the sensitive detection and quantification of enzymatic activity. It is primarily utilized by researchers and manufacturers in the life sciences, molecular biology, and diagnostic kit development sectors.

Application

• Enzyme Activity Assays: As a chromogenic substrate for the detection and kinetic study of β-galactosidase activity in vitro.
• Molecular Biology Research: Used in reporter gene assays (e.g., lacZ gene expression) to monitor gene regulation and promoter activity.
• Diagnostic Development: A key component in the formulation of diagnostic kits for bacterial detection (e.g., E. coli) and other enzymatic markers.
• High-Throughput Screening (HTS): Employed in pharmaceutical and biotechnology research for screening enzyme inhibitors or activators.
• Cell & Tissue Staining: Used in histochemistry and cytochemistry for visualizing β-galactosidase expression in cells and tissues.
• Biochemical Reagent: Serves as a standard and reference material in quality control for enzyme preparations and related products.

Basic Information

Product Name	3,4-Dinitrophenyl-β-Galactopyranoside
CAS No.	85668-54-8
Molecular Formula	C12H14N2O10
Molecular Weight	346.25 g/mol
Synonyms	3,4-Dinitrophenyl β-D-galactopyranoside; DNP-β-Gal; 3,4-DNP-β-Gal; β-D-Galactopyranoside, 3,4-dinitrophenyl; 4-Nitrophenyl β-D-galactopyranoside derivative; Galactopyranoside, 3,4-dinitrophenyl-; Chromogenic β-galactosidase substrate; Fluorogenic galactosidase substrate
EINECS	Contact for details

Quality Control

Our 3,4-Dinitrophenyl-β-Galactopyranoside is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency, suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in enzymatic assays. We adhere to relevant industry standards for biochemical reagents.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Enzymatic Activity	Suitable for β-galactosidase assays
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.