Description

Hemoglobin F Columbus-Ga is a specific variant of fetal hemoglobin (HbF), a critical protein complex for oxygen transport. This high-purity biochemical is essential for advanced research and diagnostic applications requiring precise molecular characterization. It is primarily utilized by biotechnology firms, clinical research laboratories, and diagnostic kit manufacturers focused on hemoglobinopathies, developmental biology, and hematological studies.

Application

• Biomedical Research: Used as a standard or reagent in studies of fetal hemoglobin function, oxygen affinity, and sickle cell disease mechanisms.
• Diagnostic Development: Serves as a critical reference material in the calibration and validation of diagnostic assays for detecting abnormal hemoglobin variants.
• Therapeutic Research: Investigated in preclinical studies aimed at reactivating fetal hemoglobin production as a therapeutic strategy for β-hemoglobin disorders.
• Biochemical Analysis: Employed in chromatography (HPLC, CE) and spectrometry (MS) for method development and quality control in clinical laboratories.
• Enzyme & Binding Studies: Utilized to study protein-protein interactions, ligand binding kinetics, and allosteric regulation within the hemoglobin tetramer.
• Educational & Reference Standards: Provides a purified component for academic teaching and the establishment of certified reference materials (CRMs).

Basic Information

Product Name	Hemoglobin F Columbus-Ga
CAS No.	85205-42-1
Molecular Formula	Contact for details
Molecular Weight	~64,500 Da (for tetramer)
Synonyms	HbF Columbus-Ga; Fetal Hemoglobin Columbus-Ga; γ-Globin Variant Columbus-Ga; Hemoglobin F (Columbus-Ga); Gly->Asp substitution at gamma 80; HbF Col Ga; Hb F Columbus, Georgia; Fetal Hb variant
EINECS	Contact for details

Quality Control

Our Hemoglobin F Columbus-Ga is produced and handled under stringent quality management protocols. Each batch undergoes comprehensive analytical testing to ensure identity, purity, and biological activity. A detailed Certificate of Analysis (COA) is provided, documenting key parameters such as purity by HPLC, spectral properties, and functional integrity. We adhere to ISO 13485 principles for quality management, supporting its use in research and diagnostic development.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (HPLC/CE)	Conforms to HbF Columbus-Ga standard
Purity (HPLC)	≥ 95% (HbF Columbus-Ga)
Assay (Spectrophotometry)	≥ 90% of theoretical heme content
Solubility	Clear solution in appropriate buffer
Endotoxin	< 1.0 EU/mg (for research-grade)
Biological Activity (O2 binding)	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.