Description

n-4-Azido 2-Nitrophenyl γ-Aminobutyryl-5-Adenylyl Imidodiphosphate CAS NO 84625-58-1 is a specialized, photo-activatable nucleotide analog designed for advanced biochemical research. This compound matters as a critical tool for irreversible cross-linking and affinity labeling studies, enabling precise investigation of ATP-binding sites and enzyme mechanisms. It is primarily needed by research scientists and pharmaceutical developers in the fields of enzymology, structural biology, and proteomics for studying nucleotide-dependent processes.

Application

• Enzyme Active Site Labeling: Used as an affinity label to covalently modify and identify ATP-binding pockets in kinases, ATPases, and other nucleotide-binding proteins.
• Photocrosslinking Studies: The aryl azide group allows for UV light-induced crosslinking to capture transient protein-ligand or protein-protein interactions.
• Biochemical Probe Development: Serves as a key intermediate in synthesizing probes for studying energy metabolism and signal transduction pathways.
• Structural Biology: Aids in mapping binding sites and understanding substrate specificity through irreversible inhibition.
• Proteomics Research: Employed in activity-based protein profiling (ABPP) to identify and characterize novel ATP-utilizing enzymes.
• Drug Discovery: Used in target validation and mechanistic studies for developing inhibitors of nucleotide-dependent enzymes.

Basic Information

Product Name	n-4-Azido 2-Nitrophenyl γ-Aminobutyryl-5-Adenylyl Imidodiphosphate
CAS No.	84625-58-1
Molecular Formula	C₂₁H₂₈N₁₀O₁₇P₃
Molecular Weight	809.41 g/mol
Synonyms	ANP-GABA-ATP; 5'-[4-[3-(2-Nitro-4-azidophenyl)propionamido]butyl]imidodiphosphate; NAPP-GABA-ATP; 2-Nitro-4-azidophenylaminobutyryl imidodiphosphate; γ-Aminobutyryl-ANP-ATP; Photoaffinity ATP analog; ANP-ε-aminocaproyl-ATP (common naming variant); 8-Azido-ATP derivative
EINECS	Contact for details

Quality Control

Our production of n-4-Azido 2-Nitrophenyl γ-Aminobutyryl-5-Adenylyl Imidodiphosphate adheres to stringent quality protocols suitable for research applications. Every batch is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to ensure high purity and structural integrity. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are provided to guarantee reproducible and reliable performance in your critical experiments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. Due to its hygroscopic and light-sensitive nature, the product should be handled under inert atmosphere when possible and aliquoted to minimize freeze-thaw cycles. Keep away from strong acids and bases.

Specification

Item	Specification
Appearance	Yellow to orange lyophilized powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.