Description

Sephacryl S 500 is a high-resolution, cross-linked beaded matrix designed for size exclusion chromatography (SEC) and gel filtration. This product is essential for the precise separation and purification of large biomolecules, including proteins, polysaccharides, and nucleic acids, based on their hydrodynamic size. It is a critical tool for researchers and process development scientists in the biotechnology, pharmaceutical, and life sciences industries who require reliable, high-performance separation media for downstream processing and analytical applications.

Application

• Biopharmaceutical Purification: Final polishing and buffer exchange steps for monoclonal antibodies, vaccines, and other therapeutic proteins.
• Virus and Large Protein Complex Separation: Size-based fractionation of viral particles, plasmids, and protein aggregates in process development.
• Polysaccharide and Polymer Analysis: Molecular weight distribution analysis of dextrans, pullulans, and other high molecular weight polymers.
• Desalting and Buffer Exchange: Rapid removal of salts, dyes, or other small molecules from protein and nucleic acid samples.
• Process Development and Scale-Up: From laboratory-scale method development to pilot and production-scale chromatography column packing.
• Quality Control (QC) Laboratories: Analytical gel filtration for assessing product purity, aggregation state, and stability of biomolecules.

Basic Information

Product Name	Sephacryl S 500
CAS No.	84593-65-7
Molecular Formula	Cross-linked copolymer of allyl dextran and N,N'-methylenebisacrylamide
Molecular Weight	N/A (Macromolecular matrix)
Synonyms	Sephacryl S-500; Sephacryl S500 HR; Sephacryl S-500 High Resolution; Allyl dextran-N,N'-methylenebisacrylamide copolymer; Size exclusion chromatography matrix; Gel filtration medium; SEC resin; Cross-linked dextran/bisacrylamide gel
EINECS	Contact for details

Quality Control

Our Sephacryl S 500 is manufactured under a quality management system and undergoes rigorous testing to ensure batch-to-batch consistency and performance. Key parameters such as particle size distribution, exclusion limits, flow characteristics, and chemical stability are verified. A Certificate of Analysis (COA) detailing the specifications and test results for each lot is available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). The product is supplied as a suspension in an aqueous preservative solution; do not allow the matrix to dry out. For long-term storage, ensure the container is sealed to prevent evaporation and microbial contamination.

Specification

Item	Specification
Matrix	Cross-linked allyl dextran with N,N'-methylenebisacrylamide
Particle Size	20-80 μm (dry bead diameter)
Separation Range (Globular Proteins)	40,000 - 20,000,000 Da
Exclusion Limit (Dextrans)	> 20,000,000 Da
Maximum Operating Pressure	0.3 MPa (3 bar, 44 psi)
pH Stability (operational)	2 - 13
pH Stability (long-term)	3 - 11
Chemical Stability	Stable in common aqueous buffers, 1M NaOH, 8M urea, 6M guanidine HCl, and 70% ethanol
Preservative	20% Ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.