Description

4-Methylumbelliferyl β-Glucuronide is a high-purity fluorogenic substrate used for the detection and quantification of β-glucuronidase enzyme activity. This compound is critical for research and diagnostic applications where precise enzymatic measurement is required. It is primarily utilized by biotechnology, pharmaceutical, and clinical diagnostic laboratories for enzyme-linked assays, microbial detection, and reporter gene studies.

Application

• Enzymatic Assays: As a specific fluorogenic substrate for the detection and kinetic study of β-glucuronidase (GUS) activity in vitro.
• Microbiology & Cell Biology: For identifying and enumerating E. coli and other β-glucuronidase-positive bacteria in food, water, and environmental samples.
• Reporter Gene Systems: A key reagent in molecular biology for GUS reporter gene assays to monitor gene expression and promoter activity in plant and animal tissues.
• Diagnostic Kits: An essential component in clinical and veterinary diagnostic kits designed to detect specific bacterial infections.
• Drug Metabolism Studies: Used in research to study glucuronidation pathways and the activity of UDP-glucuronosyltransferase enzymes.
• High-Throughput Screening (HTS): Employed in pharmaceutical research for screening inhibitors or activators of β-glucuronidase in drug discovery pipelines.

Basic Information

Product Name	4-Methylumbelliferyl β-Glucuronide
CAS No.	84434-84-4
Molecular Formula	C16H16O9
Molecular Weight	352.29 g/mol
Synonyms	4-MUG; 4-Methylumbelliferyl β-D-Glucuronide; 4-Methylumbelliferyl-β-D-glucuronide; β-Glucuronidase Substrate; MUG; 4-MU-β-D-Glucuronide; 7-Hydroxy-4-methylcoumarin β-D-glucuronide; GUS Substrate
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl β-Glucuronide is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzymatic assays. We ensure compliance with relevant quality benchmarks for research and in-vitro diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a desiccator before opening to prevent moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Fluorogenic Activity	Suitable for enzyme assays
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.