Description

3-(2-Bromoacetamidomethyl)-2,2,5,5-Tetramethyl-1-Pyrrolidinoxy is a stable nitroxide radical derivative, specifically a spin-labeled reagent containing a reactive bromoacetamide group. This compound is a critical tool for site-directed spin labeling (SDSL) in electron paramagnetic resonance (EPR) spectroscopy, enabling the study of protein structure, dynamics, and conformational changes. It is essential for researchers and scientists in structural biology, biophysics, and pharmaceutical development who require precise molecular probes for advanced spectroscopic analysis.

Application

• Site-Directed Spin Labeling (SDSL): Primary application for covalent attachment to cysteine residues in proteins and peptides for EPR/ESR studies.
• Protein Structure & Dynamics Analysis: Used to investigate protein folding, conformational changes, and macromolecular interactions.
• Membrane Protein Studies: Valuable for probing the structure and environment of membrane-embedded proteins.
• Nucleic Acid Labeling: Can be used to modify synthetic oligonucleotides for studying nucleic acid structure and protein-nucleic acid complexes.
• Polymer & Material Science: Acts as a spin probe for studying polymer chain dynamics, morphology, and free radical processes.
• Drug Discovery Research: Employed in fragment-based drug discovery and to study ligand-binding events using EPR techniques.

Basic Information

Product Name	3-(2-Bromoacetamidomethyl)-2,2,5,5-Tetramethyl-1-Pyrrolidinoxy
CAS No.	84233-52-3
Molecular Formula	C11H20BrN2O2
Molecular Weight	289.19 g/mol
Synonyms	3-(2-Bromoacetamidomethyl)-2,2,5,5-tetramethylpyrrolidine-1-oxyl; 3-(Bromoacetamidomethyl)-PROXYL; 3-(2-Bromoacetamido)-2,2,5,5-tetramethyl-1-pyrrolidinyloxy; Bromoacetamido-PROXYL; 3-Bromoacetamidomethyl-PROXYL; PROXYL-bromoacetamide; (2,2,5,5-Tetramethyl-1-oxyl-pyrrolidin-3-ylmethyl)-bromoacetamide; Spin label reagent
EINECS	Contact for details

Quality Control

Our 3-(2-Bromoacetamidomethyl)-2,2,5,5-Tetramethyl-1-Pyrrolidinoxy is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, critical for sensitive spectroscopic applications. Each lot is characterized by multiple analytical techniques including HPLC, NMR, and EPR spectroscopy. Certificates of Analysis (COA) detailing purity, identity, and radical content are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) due to its hygroscopic and light-sensitive nature. Allow the sealed container to reach room temperature before opening to minimize moisture condensation. Keep away from strong reducing agents.

Specification

Item	Specification
Appearance	Orange to red crystalline powder
Identification (IR)	Conforms to structure
Identification (EPR)	Characteristic triplet signal
Purity (HPLC)	≥ 95%
Radical Content (EPR)	≥ 90%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.