Description

n-α-Benzoyl-L-Arginine 7-Amido-4-Methylcoumarin Hydrochloride is a specialized fluorogenic substrate used for the sensitive detection and quantification of trypsin-like protease activity. This compound matters for researchers and quality control labs requiring precise, high-throughput enzymatic assays due to its release of a highly fluorescent product upon cleavage. It is primarily needed by the pharmaceutical, biotechnology, and diagnostic industries for drug discovery, biochemical research, and enzyme characterization.

Application

• Protease Activity Assays: A key fluorogenic substrate for the continuous kinetic measurement of trypsin, thrombin, plasmin, and other serine proteases.
• Drug Discovery & High-Throughput Screening (HTS): Enables the identification and characterization of protease inhibitors in pharmaceutical research.
• Biochemical Research: Used in enzyme kinetics studies, mechanism of action analysis, and purity assessment of protease preparations.
• Diagnostic Development: Serves as a critical component in developing clinical assays for conditions related to abnormal protease activity.
• Quality Control in Biotechnology: Employed to monitor and validate the activity of recombinant proteases during production.

Basic Information

Product Name	n-α-Benzoyl-L-Arginine 7-Amido-4-Methylcoumarin Hydrochloride
CAS No.	83701-04-6
Molecular Formula	C26H31N7O5 • HCl
Molecular Weight	582.04 g/mol
Synonyms	Benzoyl-L-arginine 7-amido-4-methylcoumarin hydrochloride; Bz-Arg-AMC • HCl; Bz-Arg-AMC hydrochloride; Bz-Arg-AMC HCL; Bz-Arg-AMC; Boc-L-Arg-AMC HCl (common misnomer); L-BAPA-AMC HCl; 7-Amido-4-methylcoumarin conjugate of N-α-Benzoyl-L-arginine hydrochloride
EINECS	Contact for details

Quality Control

Our n-α-Benzoyl-L-Arginine 7-Amido-4-Methylcoumarin Hydrochloride is manufactured under strict quality systems to ensure high purity and consistent performance in sensitive assays. Each batch is tested to meet rigorous in-house specifications, with comprehensive Certificates of Analysis (COA) provided. We support research and production needs with a commitment to reliability and traceability.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This material is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after use to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Fluorogenic Activity	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.