Description

3-(4-Biotinoyl-6-Aminocaproyloxy)Phenylpropionic Acid n-Hydroxysuccinimide Ester is a high-purity, heterobifunctional crosslinking reagent designed for bioconjugation. This compound is critically important for introducing biotin tags onto proteins, antibodies, and other biomolecules, enabling their detection, purification, and analysis via the strong streptavidin-biotin interaction. It is primarily needed by researchers and manufacturers in the life sciences, diagnostics, and pharmaceutical development sectors for applications in assay development, targeted drug delivery, and proteomics.

Application

• Bioconjugation and Labeling: Covalent attachment of biotin to primary amines (-NH2) on proteins, peptides, and antibodies for use in immunoassays (ELISA, Western Blot).
• Diagnostic Kit Manufacturing: Production of biotinylated capture antibodies and detection reagents for clinical and research diagnostic platforms.
• Affinity Purification: Creation of biotin-tagged ligands for immobilization on streptavidin or avidin resins to purify target molecules.
• Cell Surface Labeling: Tagging of cell surface proteins for fluorescence-activated cell sorting (FACS) and microscopy studies using streptavidin-fluorophore conjugates.
• Drug Delivery System Development: Conjugation of targeting ligands (e.g., antibodies) to drug carriers (e.g., liposomes, nanoparticles) via biotin-streptavidin bridges.
• Microarray and Biosensor Fabrication: Immobilization of biomolecules on sensor surfaces for high-throughput screening and detection.
• Pull-Down Assays: Investigation of protein-protein interactions by biotinylating a bait protein for capture with streptavidin beads.

Basic Information

Product Name	3-(4-Biotinoyl-6-Aminocaproyloxy)Phenylpropionic Acid n-Hydroxysuccinimide Ester
CAS No.	83592-07-8
Molecular Formula	C30H40N4O8S
Molecular Weight	616.73 g/mol
Synonyms	Biotin-XX, SSE; Biotinamidocaproyl-6-aminohexanoic acid N-hydroxysuccinimide ester; Biotin-LC-NHS; Biotin (long chain) NHS ester; N-Succinimidyl 6-((6-((biotinoyl)amino)hexanoyl)amino)hexanoate; 6-((6-(5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)hexanoic acid N-hydroxysuccinimide ester; Biotin-XX-NHS; EZ-Link Biotin-XX, NHS Ester
EINECS	Contact for details

Quality Control

Our 3-(4-Biotinoyl-6-Aminocaproyloxy)Phenylpropionic Acid n-Hydroxysuccinimide Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (NMR, MS), to ensure it meets the high standards required for sensitive life science applications. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and the active NHS ester group is susceptible to hydrolysis. For long-term stability, store under inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.