Description

Serum Spreading Factor CAS NO 83380-82-9 is a high-purity biochemical reagent, also known as Fibronectin, which plays a critical role in cell adhesion, migration, and wound healing processes. Its primary value lies in its ability to promote cell attachment and spreading on various substrates, making it an essential component in advanced cell culture and biomedical research. This product is vital for researchers and manufacturers in the biotechnology, pharmaceutical development, and diagnostic sectors who require reliable, high-quality extracellular matrix proteins for their work.

Application

• Cell Culture & Tissue Engineering: Used as a coating agent to enhance the attachment and growth of anchorage-dependent cells, including stem cells, primary cells, and established cell lines.
• Biomedical Research: Essential for studies investigating cell-matrix interactions, cell migration, wound healing mechanisms, and embryonic development.
• Diagnostic Assay Development: Serves as a substrate in enzyme-linked immunosorbent assays (ELISA) and other immunoassays for detecting antibodies or studying protein-protein interactions.
• Drug Discovery & Screening: Utilized in high-throughput screening platforms to evaluate compounds that affect cell adhesion, proliferation, or signaling pathways.
• Biomaterial & Medical Device Coating: Applied to surfaces of implants, scaffolds, and biosensors to improve biocompatibility and host tissue integration.

Basic Information

Product Name	Serum Spreading Factor
CAS No.	83380-82-9
Molecular Formula	Contact for details
Molecular Weight	~440 kDa (multimeric glycoprotein)
Synonyms	Fibronectin; Cold-Insoluble Globulin (CIG); Cell-Surface Protein; LETS Protein (Large External Transformation-Sensitive); Galactoprotein A; Anti-gelatin Factor; Uroporphyrinogen Decarboxylase Co-factor; Serum Spreading Factor; FN
EINECS	Contact for details

Quality Control

Our Serum Spreading Factor is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and high biological activity. Each lot is characterized for purity, identity, and functional performance. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specifications such as protein concentration, purity by SDS-PAGE, sterility, endotoxin levels, and biological activity in cell attachment assays. We adhere to relevant guidelines for research-grade biochemicals.

Storage

Preserve in a tightly closed container, protected from light. Store lyophilized material at 2-8°C. For reconstituted solutions, aliquot and store at -20°C or below to prevent repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after use to prevent moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear solution
Identification (IR / Electrophoresis)	Conforms to standard
Protein Content (Biuret / BCA)	> 95%
Purity (SDS-PAGE, reduced)	> 90% (under non-reducing conditions)
Biological Activity (Cell Attachment)	Promotes attachment of standard fibroblast cells at ≤ 5 µg/cm²
Endotoxin (LAL)	< 1.0 EU/mg
Sterility (Membrane Filtration)	Sterile
Solubility	Soluble in water or buffer, forming a clear, slightly opalescent solution

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.