Description

Chapso CAS NO 82473-24-3 is a high-purity, zwitterionic detergent widely recognized for its exceptional ability to solubilize membrane proteins while maintaining their native structure and function. This compound is critical for researchers and manufacturers requiring stable protein preparations for downstream analysis and bioprocessing. It is an indispensable tool in structural biology, pharmaceutical research, and diagnostic assay development, where protein integrity is paramount.

Application

• Membrane Protein Solubilization: Extraction and stabilization of integral membrane proteins from cellular membranes for biochemical and biophysical studies.
• Structural Biology: Preparation of protein samples for techniques such as X-ray crystallography, cryo-electron microscopy (cryo-EM), and nuclear magnetic resonance (NMR) spectroscopy.
• Drug Discovery & Development: Used in assays for membrane-bound drug targets, including G protein-coupled receptors (GPCRs) and ion channels.
• Diagnostic Kit Manufacturing: Formulation component for immunoassays and other diagnostic tests that require gentle detergent conditions.
• Chromatography: As an additive in purification protocols, particularly for membrane proteins, to prevent aggregation and maintain solubility.
• Cell Lysis: Component of lysis buffers for the gentle release of intracellular contents while preserving protein complexes.

Basic Information

Product Name	Chapso
CAS No.	82473-24-3
Molecular Formula	C32H58N2O7S
Molecular Weight	614.88 g/mol
Synonyms	3-[(3-Cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate; CHAPSO; 3-[(3-Cholamidopropyl)dimethylammonio]-2-hydroxypropanesulfonate; N,N-Dimethyl-3-sulfo-N-[3-(3α,7α,12α-trihydroxy-24-oxocholan-24-yl)aminopropyl]propanaminium inner salt; Zwittergent 3-12
EINECS	Contact for details

Quality Control

Our Chapso is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and rigorous tests for critical impurities like heavy metals and residual solvents. Certificates of Analysis (COA) detailing all specifications and test results are available upon request to ensure full traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive). Keep the container tightly sealed in a desiccated environment to prevent absorption of atmospheric moisture, which can affect performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
pH (1% Solution)	6.0 - 8.0
Heavy Metals (as Pb)	≤ 10 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.