Description

Nitrile Hydratase CAS NO 82391-37-5 is a highly specialized metalloenzyme that catalyzes the hydration of nitriles to their corresponding amides. This biocatalyst is critical for enabling efficient, selective, and environmentally friendly chemical transformations under mild conditions. It is an essential tool for industries focused on fine chemical synthesis, pharmaceutical intermediates, and the production of high-value amides, offering a superior alternative to traditional harsh chemical hydrolysis methods.

Application

• Acrylamide Production: The primary industrial-scale application for the enzymatic synthesis of acrylamide from acrylonitrile.
• Pharmaceutical Intermediates: Synthesis of chiral and non-chiral amide intermediates for active pharmaceutical ingredients (APIs).
• Fine Chemical Manufacturing: Production of specialty amides, including nicotinamide and other vitamin derivatives.
• Agrochemical Synthesis: Key step in the manufacture of herbicides and fungicides containing amide functional groups.
• Bioremediation: Used in enzymatic processes for the detoxification of nitrile-containing industrial waste streams.
• Research & Development: A valuable biocatalyst in organic synthesis and metabolic pathway studies within academic and industrial R&D labs.

Basic Information

Product Name	Nitrile Hydratase
CAS No.	82391-37-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	NHase; Nitrile Hydratase Enzyme; Nitrile Hydratase (EC 4.2.1.84); Nitrile Hydratase (Fe-type); Nitrile Hydratase (Co-type); Nitrile Hydratase (Low-molecular-mass); Acrylamide-producing Enzyme
EINECS	Contact for details

Quality Control

Our Nitrile Hydratase is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and optimal catalytic activity. Each lot is characterized for specific activity, purity, and stability. A comprehensive Certificate of Analysis (COA) detailing assay results, protein content, and storage stability is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term stability, consider storage at -20°C. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	≥ [Value] U/mg protein
Protein Content (Bradford)	≥ [Value] %
Purity (SDS-PAGE)	≥ [Value] %
pH (in solution)	6.0 - 8.0
Residual Moisture (for powder)	≤ [Value] %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.