Description

n-Succinimidyl 6-(2,4-Dinitroanilino)Hexanoate is a heterobifunctional crosslinking reagent featuring an amine-reactive N-hydroxysuccinimide (NHS) ester and a UV-absorbing 2,4-dinitroaniline (DNP) moiety. This compound is engineered for the efficient, site-specific labeling and conjugation of biomolecules, enabling downstream detection and purification. It is primarily utilized by researchers and manufacturers in the fields of bioconjugation, proteomics, and immunoassay development, where reliable and stable linker chemistry is critical.

Application

• Antibody-Drug Conjugate (ADC) Linker Chemistry: Serves as a key intermediate for constructing cleavable or non-cleavable linkers connecting cytotoxic payloads to monoclonal antibodies.
• Protein and Peptide Labeling: Facilitates the covalent attachment of the DNP hapten to primary amines (e.g., lysine residues) for detection using anti-DNP antibodies in ELISA, Western blotting, and immunofluorescence.
• Affinity Purification Tagging: Used to introduce a DNP tag onto proteins of interest for purification via anti-DNP affinity columns.
• Surface Immobilization: Enables the functionalization of sensor chips, beads, or other solid supports with DNP groups for capturing specific antibodies or other binding partners in biosensor and diagnostic applications.
• Biomolecular Crosslinking Studies: Acts as a tool compound for investigating protein-protein interactions or creating defined molecular complexes for structural analysis.
• Diagnostic Reagent Synthesis: A building block in the production of reagents for clinical diagnostic kits that rely on hapten-based signal amplification systems.

Basic Information

Product Name	n-Succinimidyl 6-(2,4-Dinitroanilino)Hexanoate
CAS No.	82321-04-8
Molecular Formula	C18H21N5O8
Molecular Weight	435.39 g/mol
Synonyms	6-[(2,4-Dinitrophenyl)amino]hexanoic Acid N-Succinimidyl Ester; NHS-DNP; N-Succinimidyl 6-(2,4-DNP-amino)hexanoate; DNP-X, SE; 2,5-Dioxopyrrolidin-1-yl 6-[(2,4-dinitrophenyl)amino]hexanoate; N-Succinimidyl-6-(2,4-dinitroanilino)hexanoate; DNP Aminocaproic Acid NHS Ester
EINECS	Contact for details

Quality Control

Our n-Succinimidyl 6-(2,4-Dinitroanilino)Hexanoate is manufactured under strict quality systems to ensure batch-to-batch consistency and high reactivity. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and functional activity validation. Certificates of Analysis (COA) detailing all specifications are provided to support your research and cGMP manufacturing requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.