Description

Pertussis Toxin is a key virulence factor and exotoxin produced by the bacterium *Bordetella pertussis*, the causative agent of whooping cough. This high-purity biological reagent is essential for advanced research in immunology, vaccine development, and cellular signaling studies. It is primarily utilized by pharmaceutical R&D departments, academic research institutions, and biotechnology companies focused on respiratory diseases and G-protein coupled receptor (GPCR) pathways.

Application

• Immunological Research: Critical for studying the pathogenesis of whooping cough, host immune responses, and the development of acellular pertussis vaccines.
• Cell Signaling Studies: Widely used as a specific and irreversible activator of inhibitory G proteins (Gi/o), making it a fundamental tool in GPCR and adenylate cyclase research.
• Vaccine Potency Testing: Employed in the quality control and standardization of pertussis vaccines to ensure efficacy and safety.
• Autoimmune Disease Models: Used to induce experimental autoimmune encephalomyelitis (EAE) in animal models for multiple sclerosis research.
• Biochemical Assay Development: Serves as a standard and reagent in enzymatic assays to study ADP-ribosylation.
• Toxicology & Pharmacology: Applied in studies investigating the effects of bacterial toxins on cellular function and potential therapeutic interventions.

Basic Information

Product Name	Pertussis Toxin
CAS No.	82248-93-9
Molecular Formula	Contact for details
Molecular Weight	Approx. 105,000 Da (Hexamer)
Synonyms	Pertussis Toxin; PTX; Islet-Activating Protein; IAP; Lymphocytosis-Promoting Factor; LPF; Pertussigen; Bordetella pertussis toxin; Whooping cough toxin
EINECS	Contact for details

Quality Control

Our Pertussis Toxin is produced and handled under stringent conditions to ensure batch-to-batch consistency and biological activity. Each lot undergoes comprehensive analytical testing, including activity assays (e.g., CHO cell clustering, ADP-ribosyltransferase activity), purity assessment via SDS-PAGE, and sterility testing. A detailed Certificate of Analysis (COA) documenting purity, activity (in µg or ng), endotoxin levels, and storage buffer is provided with every shipment to support your regulatory and research requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C to -80°C. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the vial is equilibrated to room temperature before opening to prevent condensation. Handle in accordance with laboratory safety procedures for biological toxins.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear solution
Identification (SDS-PAGE)	Characteristic band pattern for S1-S5 subunits
Purity (SDS-PAGE)	> 95%
Biological Activity	Defined by CHO cell clustering or ADP-ribosyltransferase assay
Protein Content (Bradford/Lowry)	As per COA
Endotoxin Level (LAL)	< 1.0 EU/µg
Sterility	Sterile filtered or tested
Buffer Composition	Typically PBS or Tris buffer, pH ~7.4

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.