Description

Endoproteinase Arg-C is a highly specific serine protease that cleaves peptide bonds at the C-terminal side of arginine residues. This precise enzymatic activity makes it an indispensable tool for protein sequencing, peptide mapping, and structural analysis in advanced biochemical research. It is essential for scientists and manufacturers in the biotechnology, pharmaceutical, and proteomics industries who require high-fidelity protein digestion for mass spectrometry, antibody characterization, and recombinant protein analysis.

Application

• Peptide Mapping & Protein Sequencing: Primary tool for generating specific peptide fragments for mass spectrometric analysis and N-terminal sequencing.
• Biopharmaceutical Characterization: Critical for analyzing monoclonal antibodies, fusion proteins, and other therapeutic biologics to assess primary structure, post-translational modifications, and lot-to-lot consistency.
• Proteomics Research: Used in bottom-up proteomics workflows for the specific digestion of complex protein mixtures prior to LC-MS/MS analysis.
• Structural Biology Studies: Aids in domain mapping, epitope identification, and the study of protein-protein interactions by creating defined cleavage products.
• Recombinant Protein Analysis: Verifies the integrity and correct processing of expressed proteins, including checking for the presence of arginine-rich tags or linkers.
• Food & Feed Protein Analysis: Employed in the specialized analysis of protein content and composition in high-value nutritional products.

Basic Information

Product Name	Endoproteinase Arg-C
CAS No.	82047-85-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Arg-C Protease; Arginine-specific Protease; Clostripain (from *Clostridium histolyticum*); Endoproteinase Arg-C; Proteinase Arg-C; Arg-C Endopeptidase; Clostripain; EC 3.4.22.8
EINECS	Contact for details

Quality Control

Our Endoproteinase Arg-C is produced and tested under stringent quality management systems. Each lot is characterized for specific activity, purity, and absence of non-specific proteolytic activities. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specifications for activity, protein content, and stability. Our quality commitment ensures the reagent performs consistently in your most sensitive analytical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This material is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Lyophilized powder
Identification (Activity)	Cleaves C-terminal to Arg residues
Specific Activity	> 100 units/mg protein
Purity (SDS-PAGE)	> 90%
Protease, Free (Assay)	None detected
Endoproteinase Lys-C Activity	None detected
Trypsin Activity	None detected
Chymotrypsin Activity	None detected
Solubility	Soluble in water or appropriate buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.