Description

tert-Butyloxycarbonyl-Norleucyl-Glutaminyl-Leucyl-Glycyl-Arginine-7-Amino-4-Methylcoumarin is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and developers requiring precise, real-time detection of protease activity, particularly in complex biological matrices. It is primarily utilized in pharmaceutical R&D, biochemical research, and diagnostic assay development for studying enzyme kinetics and inhibitor screening.

Application

• Protease Activity Assays: As a fluorogenic substrate for the continuous, real-time monitoring of specific protease enzyme activity.
• Pharmaceutical Research & Development: For high-throughput screening (HTS) of potential therapeutic protease inhibitors in drug discovery pipelines.
• Biochemical Mechanism Studies: In kinetic studies to determine enzyme parameters such as Km and Vmax for fundamental research.
• Diagnostic Kit Development: As a key component in the development of sensitive in-vitro diagnostic (IVD) tests for clinical analysis.
• Cell Biology & Signal Transduction Research: To investigate protease involvement in cellular processes like apoptosis and signal transduction pathways.
• Quality Control in Biologics Manufacturing: For monitoring and validating the activity of protease enzymes used in bioprocessing.

Basic Information

Product Name	tert-Butyloxycarbonyl-Norleucyl-Glutaminyl-Leucyl-Glycyl-Arginine-7-Amino-4-Methylcoumarin
CAS No.	81943-91-1
Molecular Formula	C45H72N12O13
Molecular Weight	965.13 g/mol
Synonyms	Boc-Nle-Gln-Leu-Gly-Arg-AMC; Boc-Norleucyl-Glutaminyl-Leucyl-Glycyl-Arginine-AMC; Fluorogenic Substrate for Protease; AMC-labeled Peptide Substrate; t-Butyloxycarbonyl-Nle-Gln-Leu-Gly-Arg-7-Amino-4-Methylcoumarin; Protease Substrate 81943-91-1; Peptide-AMC Conjugate; Arg-AMC Substrate
EINECS	Contact for details

Quality Control

Our tert-Butyloxycarbonyl-Norleucyl-Glutaminyl-Leucyl-Glycyl-Arginine-7-Amino-4-Methylcoumarin is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for critical research applications. Each lot undergoes comprehensive analytical characterization, including purity verification by HPLC and structural confirmation. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting identity, purity, and other critical parameters.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. Due to its hygroscopic nature, the product should be allowed to equilibrate to room temperature in a desiccator before opening to prevent moisture absorption. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.