Description

4-Hydroxyestrone-1,2,16,16-D4 is a high-purity, deuterium-labeled internal standard critical for ensuring analytical accuracy and reliability in quantitative mass spectrometry. This stable isotope-labeled compound is essential for researchers and quality control laboratories requiring precise measurement of endogenous estrogens and their metabolites. It is primarily used in pharmaceutical research, clinical diagnostics, and endocrinology studies for method development and validation.

Application

• Quantitative Mass Spectrometry (LC-MS/MS, GC-MS): Serves as a critical internal standard for the accurate quantification of endogenous 4-hydroxyestrone and related estrogen metabolites in complex biological matrices.
• Clinical Research & Diagnostics: Used in developing and validating assays for hormone level monitoring, metabolic disorder studies, and cancer biomarker research.
• Pharmaceutical Development: Employed in pharmacokinetic (PK) and pharmacodynamic (PD) studies to track drug metabolism and estrogen-related compound disposition.
• Endocrinology & Toxicology Studies: Facilitates research into estrogen metabolism pathways, endocrine disruption, and the role of catechol estrogens in disease.
• Method Development & Validation: Provides a reference for establishing specificity, accuracy, and precision in new analytical methods for steroid hormones.
• Quality Control & Reference Standards: Acts as a certified reference material (CRM) for calibrating laboratory equipment and verifying assay performance in diagnostic and testing laboratories.

Basic Information

Item	Details
Product Name	4-Hydroxyestrone-1,2,16,16-D4
CAS No.	81586-98-3
Molecular Formula	C18H18D4O3
Molecular Weight	274.38 g/mol (approx.)
Synonyms	1,2,16,16-D4-4-Hydroxyestrone; 4-Hydroxyestrone-d4; 3-Hydroxy-1,3,5(10)-estratrien-17-one-1,2,16,16-d4; Estrone-4-ol-d4; Deuterated 4-Hydroxyestrone; [1,2,16,16-2H4]-4-Hydroxyestrone; 4-OHE1-d4; 4-OH-Estrone-d4
EINECS	Contact for details

Quality Control

Our 4-Hydroxyestrone-1,2,16,16-D4 is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to ensure high chemical purity and isotopic enrichment suitable for use as an internal standard. A comprehensive Certificate of Analysis (COA) detailing purity, isotopic purity (D-enrichment), and analytical results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere for long-term stability. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-enrichment)	≥ 98 atom % D
Chemical Purity (by NMR)	Conforms
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.