Description

3,3'-Dithiobis(Sulfosuccinimidylpropionate) is a heterobifunctional crosslinking agent featuring an amine-reactive N-hydroxysulfosuccinimide (sulfo-NHS) ester group and a cleavable disulfide bond. This compound matters for its water-solubility and ability to create reversible, reducible linkages between biomolecules, which is critical for controlled conjugation and release studies. It is primarily needed by researchers and manufacturers in the life sciences, particularly for bioconjugation, antibody-drug conjugate (ADC) development, and the fabrication of stimuli-responsive biomaterials.

Application

• Bioconjugation and Protein Labeling: For covalently linking proteins, peptides, or antibodies to other molecules (e.g., enzymes, fluorophores, drugs) via primary amines, with the disulfide bond allowing for subsequent cleavage under reducing conditions.
• Antibody-Drug Conjugate (ADC) Linker Exploration: Serves as a model or functional linker in ADC research to study the efficacy of disulfide-based, traceless cleavage mechanisms for targeted drug delivery.
• Surface Modification and Immobilization: Used to functionalize surfaces (e.g., biosensor chips, nanoparticles) with biomolecules that can be released or exchanged, enabling reversible capture and analysis.
• Polymer and Biomaterial Science: A key reagent for synthesizing smart hydrogels or polymeric carriers where disulfide cleavage triggers payload release in response to intracellular glutathione levels.
• Affinity Chromatography Ligand Coupling: For attaching ligands to chromatography resins, allowing for gentle elution of bound targets using reducing agents like DTT or TCEP.
• Crosslinking for Structural Analysis: In structural biology to capture and stabilize protein-protein interactions for analysis via techniques like mass spectrometry.

Basic Information

Product Name	3,3'-Dithiobis(Sulfosuccinimidylpropionate)
CAS No.	81069-02-5
Molecular Formula	C16H20N2O14S4 · 2Na
Molecular Weight	670.57 g/mol (Disodium salt)
Synonyms	DTSSP; Bis(sulfosuccinimidyl) 3,3'-dithiodipropionate; 3,3'-Dithiobis(sulfosuccinimidyl propionate) disodium salt; Sulfo-DTSSP; Bis[sulfosuccinimidyl] 3,3'-dithiodipropionate; DTSSP, disodium salt; Bis(N-hydroxysulfosuccinimidyl) 3,3'-dithiodipropionate; L-DTSSP
EINECS	Contact for details

Quality Control

Our 3,3'-Dithiobis(Sulfosuccinimidylpropionate) is manufactured under a quality management system and undergoes rigorous analytical testing to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot is supplied with a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional suitability. We can support development and production needs in compliance with relevant cGMP and ISO 9001 standards for research and pharmaceutical-grade materials.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Solubility (Water)	Clear, colorless solution at 10 mg/mL
Functional Suitability	Conjugation efficiency confirmed by model reaction

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.