Description

Carboxypeptidase B CAS NO 80651-95-2 is a highly specific zinc-containing exopeptidase enzyme that cleaves C-terminal lysine and arginine residues from peptides and proteins. This precise biochemical activity is critical for applications requiring controlled proteolysis, such as the processing of recombinant proteins and insulin. It is an essential reagent for biopharmaceutical manufacturers, diagnostic kit developers, and research institutions focused on protein sequencing, peptide mapping, and bioprocess development.

Application

• Recombinant Protein & Insulin Purification: For the precise removal of C-terminal lysine residues from monoclonal antibodies and other therapeutic proteins to ensure product homogeneity and stability.
• Peptide Mapping & Protein Sequencing: As a specific tool in analytical biochemistry to generate defined peptide fragments for mass spectrometry analysis and structural characterization.
• Biopharmaceutical Process Development: Used in downstream processing to cleave affinity tags (e.g., poly-arginine tags) from fusion proteins.
• Diagnostic Reagent Manufacturing: A key component in enzymatic assay kits for clinical diagnostics and biochemical research.
• Academic & Industrial Research: For studying protease specificity, enzyme kinetics, and protein-protein interactions in life science research.
• Food Science Applications: Potential use in protein hydrolysate production and flavor enhancement through controlled proteolysis.

Basic Information

Product Name	Carboxypeptidase B
CAS No.	80651-95-2
Molecular Formula	Contact for details
Molecular Weight	~34 kDa (Monomer)
Synonyms	Carboxypeptidase B (Porcine); CPB; Protaminase; Peptidyl-L-lysine(-L-arginine) hydrolase; Carboxypeptidase B, Pancreatic; 3.4.17.2; Carboxypeptidase B from Porcine Pancreas; Carboxypeptidase B Enzyme
EINECS	Contact for details

Quality Control

Our Carboxypeptidase B is produced and tested under a rigorous quality management system. Each batch is characterized for specific activity, purity, and absence of contaminating protease activities to ensure consistent performance. Certificates of Analysis (COA) detailing assay results, including activity (units/mg protein), protein content, and purity by SDS-PAGE, are provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at 2-8°C. Reconstituted enzyme solutions should be aliquoted and stored at -20°C or below to prevent loss of activity. The product is hygroscopic (moisture-sensitive); ensure the container is kept sealed in a dry environment.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms
Specific Activity	≥ 100 units/mg protein
Protein Content (Biuret)	≥ 70%
Purity (SDS-PAGE)	≥ 90%
Loss on Drying	≤ 5.0 %
Solubility	Clear, colorless solution in buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.