Description

Protein Tyrosine Kinase From Bovine Spleen is a purified enzyme preparation derived from bovine tissue, specifically catalyzing the transfer of a phosphate group to tyrosine residues in protein substrates. This product is essential for biochemical research, serving as a critical reagent in signal transduction studies and kinase activity assays. It is primarily utilized by research institutions, pharmaceutical R&D departments, and diagnostic kit manufacturers for studying cellular pathways, drug discovery, and developing enzyme-linked assays.

Application

• Biochemical Research: Used as a standard enzyme in studies of phosphorylation mechanisms and signal transduction pathways.
• Drug Discovery & Screening: Acts as a target enzyme in high-throughput screening (HTS) assays to identify potential kinase inhibitors for therapeutic development.
• Diagnostic Development: Employed in the development and calibration of ELISA and other immunoassay kits that detect phosphorylated proteins.
• Enzyme Kinetics: Serves as a model enzyme for studying Michaelis-Menten kinetics, enzyme inhibition, and catalytic efficiency.
• Cell Signaling Studies: Used to phosphorylate specific substrate proteins in vitro to investigate downstream effects in cellular models.
• Academic & Educational Use: Provides a reliable enzyme source for university laboratories and teaching experiments in advanced biochemistry.

Basic Information

Product Name	Protein Tyrosine Kinase From Bovine Spleen
CAS No.	80449-02-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Bovine Spleen Protein Tyrosine Kinase; PTK (Bovine Spleen); Tyrosine-Protein Kinase (Bovine Spleen); EC 2.7.10.2 (from bovine spleen); Spleen Tyrosine Kinase homolog; Non-receptor tyrosine kinase preparation; c-Src related kinase preparation; Protein-tyrosine kinase (ATP:protein-tyrosine O-phosphotransferase)
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards for research-grade enzymes. Each batch is characterized for specific activity and purity to guarantee consistent performance in sensitive assays. Certificates of Analysis (COA) detailing assay results, purity, and storage buffer composition are available upon request. Our quality system is designed to meet the exacting requirements of life science research and development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This material is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after each use to prevent absorption of atmospheric moisture. For short-term use, aliquots may be stored at 2-8°C for up to one week. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution in buffer
Identification (Activity)	Catalyzes tyrosine phosphorylation of specific substrate
Specific Activity	≥ [Value] units/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Concentration	Determined by Bradford or UV absorbance
Buffer Composition	Tris-HCl, Glycerol, DTT, etc. (as specified per lot)
Endotoxin Level	< 1.0 EU/μg (for cell-based applications)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.