Description

Dimethyl-3,3'-Dithiobis-Succinimidylpropionate is a heterobifunctional crosslinking reagent featuring an amine-reactive NHS ester and a cleavable disulfide bond. This compound is critical for creating reversible, reducible linkages in bioconjugation strategies, enabling the study of protein interactions and the development of targeted delivery systems. It is primarily utilized by researchers and manufacturers in the fields of biopharmaceuticals, proteomics, and advanced material science for applications requiring controlled assembly and disassembly of molecular complexes.

Application

• Bioconjugation and Protein Crosslinking: For covalently linking proteins, peptides, or antibodies to other molecules (e.g., drugs, fluorophores, surfaces) via amine groups, with the disulfide bond allowing cleavage under reducing conditions.
• Antibody-Drug Conjugate (ADC) Development: As a linker in the construction of ADCs, facilitating the attachment of cytotoxic payloads while enabling intracellular release upon disulfide reduction.
• Surface Modification and Immobilization: For functionalizing nanoparticles, microspheres, or biosensor surfaces with biomolecules to create smart, responsive interfaces.
• Proteomics Research: In the study of protein-protein interactions and complex formation, where reversible crosslinking is required for analysis.
• Polymer and Biomaterial Science: As a building block for synthesizing degradable hydrogels or polymers with stimuli-responsive properties.
• Diagnostic Reagent Formulation: In the development of assay components, such as enzyme conjugates or labeled probes, for diagnostic kits.

Basic Information

Product Name	Dimethyl-3,3'-Dithiobis-Succinimidylpropionate
CAS No.	80434-78-2
Molecular Formula	C16H20N2O8S2
Molecular Weight	440.47 g/mol
Synonyms	DTSSP; 3,3'-Dithiobis(sulfosuccinimidyl propionate); Bis(sulfosuccinimidyl) 3,3'-dithiopropionate; Dithiobis(succinimidylpropionate); DSSP; Lomant's Reagent DTSSP; Succinimidyl 3-((2-(3-succinimidyl-3-oxopropoxy)ethyl)dithio)propionate; 1,1'-[Dithiobis(3-oxo-3,1-propanediyl)]bis[2,5-pyrrolidinedione] dimethyl ester
EINECS	Contact for details

Quality Control

Our Dimethyl-3,3'-Dithiobis-Succinimidylpropionate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and bioconjugation applications. Certificates of Analysis (COA) with detailed specifications are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.