Description

Alkaline Phosphatase Stop Solution is a specialized reagent formulated to rapidly and effectively terminate the enzymatic activity of alkaline phosphatase (ALP) in biochemical assays. This precise termination is critical for obtaining accurate, reproducible, and time-sensitive results in diagnostic and research applications. It is an essential component for laboratories in the in-vitro diagnostics (IVD), life science research, and pharmaceutical quality control sectors, ensuring assay integrity and data reliability.

Application

• ELISA and Immunoassay Development: Used to stop the colorimetric reaction in enzyme-linked immunosorbent assays (ELISA) utilizing alkaline phosphatase as the reporter enzyme.
• Clinical Diagnostics: Integral to automated and manual clinical chemistry analyzers for precise endpoint determination in ALP-based diagnostic test kits.
• Molecular Biology: Termination of reactions in nucleic acid detection systems, such as those using alkaline phosphatase-labeled probes (e.g., Southern, Northern, and Western blotting).
• Biochemical Research: Halting enzymatic kinetics studies to allow for accurate measurement of reaction rates and product formation over time.
• Pharmaceutical Testing: Employed in quality control assays within biomanufacturing to monitor and control processes involving alkaline phosphatase.
• Food Safety & Environmental Testing: Used in diagnostic kits for pathogen or contaminant detection that rely on ALP-conjugated antibodies.

Basic Information

Item	Detail
Product Name	Alkaline Phosphatase Stop Solution
CAS No.	80395-90-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	ALP Stop Solution; Alkaline Phosphatase Stopping Solution; Phosphatase Alkaline Stop Reagent; AP Stop Buffer; ELISA Stop Solution (for ALP); pNPP Stop Solution; Alkaline Phosphatase Reaction Terminator; Diagnostic Stop Reagent
EINECS	Contact for details

Quality Control

Our Alkaline Phosphatase Stop Solution is manufactured under a strict quality management system. Each batch undergoes rigorous performance testing to ensure it delivers consistent, rapid, and complete cessation of alkaline phosphatase activity without interfering with signal detection. We provide comprehensive Certificates of Analysis (COA) with key performance parameters, supporting compliance with ISO 13485 standards for in-vitro diagnostic reagents. Specifications are designed to meet the critical demands of diagnostic and research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at room temperature (15-25°C) in a dry, well-ventilated area. Keep away from strong bases and organic solvents. The solution is light-sensitive; prolonged exposure to light should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	Clear, colorless to slightly colored solution
Performance (Inhibition)	>99.5% inhibition of standard ALP activity within specified time
pH	As per grade requirement
Clarity	Clear by visual inspection
Bioburden	< 10 CFU/mL (for diagnostic grades)
Endotoxin	< 0.25 EU/mL (for diagnostic grades)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.