Description

p-Nitrophenyl 6-o-B-D-Galactopyranosyl-B-D-Galacto is a specialized chromogenic substrate used in enzymatic assays. This compound is critical for the detection and quantification of specific glycosidase enzyme activity, providing a reliable colorimetric readout. It is primarily needed by researchers and quality control laboratories in the pharmaceutical, diagnostic, and life science research sectors for biochemical analysis and assay development.

Application

• Enzymatic Substrate for β-Galactosidase: Used as a chromogenic substrate in assays to measure β-galactosidase activity, a common reporter gene in molecular biology.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for detecting enzymatic deficiencies or microbial contamination.
• Biochemical Research: Employed in enzyme kinetics studies, inhibitor screening, and characterization of glycosidase enzymes.
• Pharmaceutical Quality Control: Utilized in purity and potency testing of enzyme-based biopharmaceuticals.
• Food & Beverage Industry Analysis: Applied in assays to monitor fermentation processes or detect specific enzymatic activities in food products.
• Cell Biology & Reporter Assays: Serves as a substrate in histochemical staining and cell-based reporter gene assays (e.g., lacZ gene expression).

Basic Information

Product Name	p-Nitrophenyl 6-o-B-D-Galactopyranosyl-B-D-Galacto
CAS No.	80321-98-8
Molecular Formula	C18H25NO13
Molecular Weight	463.39 g/mol
Synonyms	6-O-β-D-Galactopyranosyl-β-D-galactopyranose 1-(4-nitrophenyl) derivative; p-Nitrophenyl 6-O-β-D-galactopyranosyl-β-D-galactopyranoside; 4-Nitrophenyl 6-O-β-D-galactopyranosyl-β-D-galactopyranoside; PNPG2; pNP-β-D-Gal-(1→6)-β-D-Gal; 4-Nitrophenyl β-D-galactopyranosyl-(1→6)-β-D-galactopyranoside; 1-(4-Nitrophenyl)-6-O-β-D-galactopyranosyl-β-D-galactopyranoside
EINECS	Contact for details

Quality Control

Our p-Nitrophenyl 6-o-B-D-Galactopyranosyl-B-D-Galacto is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity, spectral identification (IR, NMR), and specific activity validation in enzymatic assays. Certificates of Analysis (COA) with detailed specifications are provided to ensure compliance with research and diagnostic application standards.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. Due to its hygroscopic (moisture-sensitive) nature, the container must be kept sealed under anhydrous conditions to maintain stability and prevent decomposition.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Specific Rotation	Contact for details
Enzymatic Activity	Contact for details
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.