Description

Factor H, Human CAS NO 80295-65-4 is a high-purity, biologically active complement regulatory protein essential for controlling the alternative pathway of the complement system. Its primary value lies in its critical role as a cofactor for the factor I-mediated cleavage of C3b, preventing excessive immune activation and host tissue damage. This protein is vital for researchers and manufacturers in the fields of immunology, diagnostics, and biopharmaceutical development, particularly for studying autoimmune diseases, developing therapeutic antibodies, and producing quality control reagents.

Application

• Biopharmaceutical Research & Development: Used as a critical reagent in studying complement pathway dysregulation, target validation for autoimmune and inflammatory diseases, and screening for therapeutic inhibitors.
• Diagnostic Kit Manufacturing: Serves as a key component (antigen or calibrator) in ELISA and other immunoassays designed to detect autoimmune disorders like atypical hemolytic uremic syndrome (aHUS) or measure complement factor levels in patient serum.
• Cell Culture & In Vitro Studies: Employed in functional cell-based assays to investigate the role of complement in disease models, including those for age-related macular degeneration (AMD) and kidney diseases.
• Therapeutic Antibody Production & QC: Acts as a specificity control or blocking agent in the development and quality control of monoclonal antibodies targeting the complement system.
• Biochemical Reagent Production: Used as a purified protein source to create standardized research kits, enzyme substrates, and other specialized biochemical reagents for complement analysis.
• Academic & Institutional Research: Essential for fundamental research into innate immunity, protein-protein interactions, and the structural biology of complement regulators.

Basic Information

Product Name	Factor H, Human
CAS No.	80295-65-4
Molecular Formula	Contact for details
Molecular Weight	~155 kDa (monomer)
Synonyms	Complement Factor H; CFH; H Factor 1 (Complement); β-1H Globulin; AHUS4; ARMD4; ARMS1; CFHL3; FHL1; HF; HF1; HF2; HUS; Complement Factor H Isoform; CFH Protein, Human; Human Complement Factor H
EINECS	Contact for details

Quality Control

Our Factor H, Human is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and biological activity. Each lot undergoes comprehensive analysis, including purity assessment, functional activity testing, and confirmation of identity. A Certificate of Analysis (COA) detailing specifications such as purity (by SDS-PAGE and HPLC), endotoxin level, and functional activity is provided with every shipment to support your regulatory and research requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the vial is equilibrated to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear solution
Identification (SDS-PAGE/Western Blot)	Corresponds to standard
Purity (SDS-PAGE)	> 95%
Purity (HPLC)	> 98%
Assay (Functional Activity)	> 90% of reference standard
Endotoxin	< 1.0 EU/μg
Protein Concentration	As per COA (typically 0.5 - 1.0 mg/mL)
Buffer Composition	PBS, pH 7.4 (or as specified on COA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.