Description

Complement C5a, Human is a purified anaphylatoxin protein derived from the fifth component of the human complement system. This bioactive peptide plays a critical role in immunology research as a potent mediator of inflammation and chemotaxis. It is essential for researchers and developers in the fields of immunology, inflammation studies, and therapeutic antibody development. The product is supplied as a high-purity reagent suitable for in vitro diagnostic and research applications.

Application

• Immunological Research: Used as a key reagent to study the complement cascade, inflammatory responses, and immune cell activation (e.g., neutrophil and monocyte chemotaxis).
• Drug Discovery & Development: Serves as a target or tool compound for screening and validating potential anti-inflammatory therapeutics, including C5a receptor antagonists.
• In Vitro Diagnostics (IVD): Employed as a critical component or calibrator in ELISA kits and other immunoassays designed to measure complement activation.
• Biomarker Studies: Utilized in research investigating the role of complement activation in autoimmune diseases, sepsis, and ischemia-reperfusion injury.
• Cell Signaling Studies: Applied in research to elucidate G-protein-coupled receptor (GPCR) signaling pathways mediated by the C5a receptor (C5aR1/CD88).
• Quality Control & Standardization: Acts as a reference standard for the quality control of related biological products and assays in pharmaceutical and biotech manufacturing.

Basic Information

Product Name	Complement C5a, Human
CAS No.	80295-54-1
Molecular Formula	C254H402N72O74S6
Molecular Weight	Approx. 5,800 Da
Synonyms	C5a Anaphylatoxin; Complement C5a Fragment; Complement Component 5a; C5a Des-Arg; Human Complement C5a; C5a (Human); Complement 5a; Anaphylatoxin C5a; C5a Protein, Human; Complement Component C5a; C5a (74 aa); C5a (Homo sapiens)
EINECS	Contact for details

Quality Control

Our Complement C5a, Human is manufactured under stringent conditions to ensure high biological activity and purity. Each batch undergoes rigorous analytical testing, including SDS-PAGE, HPLC, and mass spectrometry, to confirm identity and purity. The product is tested for endotoxin levels to ensure suitability for cell-based assays. A comprehensive Certificate of Analysis (COA) detailing purity, biological activity (e.g., chemotaxis assay), and specification results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term storage, keep the lyophilized powder at -20°C or below. After reconstitution, aliquot and store at -70°C or below to maintain stability and prevent repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the vial is equilibrated to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (SDS-PAGE & HPLC)	≥ 95%
Molecular Weight	Approx. 5.8 kDa
Biological Activity (Cell-based assay)	Contact for details
Endotoxin Level	< 1.0 EU/µg
Solubility	Soluble in water or PBS

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.