Description

Complement C1R, Human, Activated, Two-Chain Form is a highly purified, activated serine protease enzyme derived from human plasma. This protein plays a critical role in the classical complement pathway, initiating the immune response cascade. It is an essential reagent for biomedical research, diagnostic assay development, and quality control in biopharmaceutical production, particularly for products targeting the complement system.

Application

• Biochemical Research: Used as a key substrate or enzyme in studies of the classical complement pathway, immune complex processing, and inflammatory mechanisms.
• Diagnostic Development: Serves as a critical component in the development and calibration of immunoassays and functional tests for complement deficiencies and autoimmune diseases.
• Drug Discovery & Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize novel inhibitors or modulators of the C1 complex for therapeutic applications.
• Biopharmaceutical QC: Acts as a reference standard or challenge agent in the quality control testing of therapeutic proteins (e.g., monoclonal antibodies) to assess potential complement activation.
• Enzymology Studies: Utilized to investigate serine protease kinetics, specificity, and regulation within complex proteolytic cascades.

Basic Information

Product Name	Complement C1R, Human, Activated, Two-Chain Form
CAS No.	80295-34-7
Molecular Formula	Contact for details
Molecular Weight	~83 kDa (Single Chain Proenzyme), ~170 kDa (Tetrameric C1 Complex)
Synonyms	Activated Complement C1r
C1R Protease
Complement Component 1, r Subcomponent, Activated Form
EC 3.4.21.41
Human C1r
Complement C1r Esterase
Serine Protease C1r
EINECS	Contact for details

Quality Control

Our Complement C1R is produced under stringent conditions to ensure high biological activity and purity. Each lot is rigorously tested for functional activity, purity profile, and the absence of contaminating proteases. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specifications such as activity units/mg, purity by SDS-PAGE, and endotoxin levels, ensuring it meets the exacting standards required for research and development applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Upon reconstitution, aliquot and store at -70°C or below to prevent loss of activity. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the vial is at room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (SDS-PAGE)	Corresponds to activated two-chain form (~83 kDa under reducing conditions)
Purity (SDS-PAGE)	≥ 90%
Specific Activity	Contact for details
Protein Content (Bradford)	Contact for details
Endotoxin (LAL)	< 1.0 EU/mg
Solubility	Soluble in buffered aqueous solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.