Description

4-Methylumbelliferyl-2-Acetamido-2-Deoxy-α-D-Glucopyranoside is a specialized fluorogenic substrate designed for the sensitive detection and quantification of enzyme activity. This compound matters because it provides a reliable and high-throughput method for biochemical research and diagnostic applications, offering a clear fluorescent signal upon enzymatic cleavage. It is primarily needed by researchers and manufacturers in the life sciences, pharmaceutical development, and clinical diagnostics sectors for studying glycosidase enzymes like α-N-acetylglucosaminidase.

Application

• Enzyme Activity Assays: A critical substrate for the fluorometric determination of α-N-acetylglucosaminidase and related glycosidase activities in research.
• Diagnostic Kit Manufacturing: Used as a key component in the production of diagnostic kits for lysosomal storage disorders, such as Sanfilippo syndrome (MPS IIIB).
• High-Throughput Screening (HTS): Enables rapid screening of potential enzyme inhibitors or activators in drug discovery pipelines.
• Biochemical Research: Employed in studies of carbohydrate metabolism, enzyme kinetics, and mechanism-of-action investigations.
• Quality Control in Biopharma: Serves as a reagent for testing the purity and activity of enzyme preparations used in therapeutic processes.
• Cell Biology Studies: Useful for assaying enzyme activity within cell lysates or in situ to understand cellular processes.

Basic Information

Product Name	4-Methylumbelliferyl-2-Acetamido-2-Deoxy-α-D-Glucopyranoside
CAS No.	80265-04-9
Molecular Formula	C18H21NO9
Molecular Weight	395.36 g/mol
Synonyms	4-MU-α-GlcNAc; 4-Methylumbelliferyl α-N-acetylglucosaminide; 4-Methylumbelliferyl N-acetyl-α-D-glucosaminide; α-4-MU-GlcNAc; 2-Acetamido-2-deoxy-α-D-glucopyranoside 4-methylumbelliferyl; N-Acetyl-α-D-glucosaminide 4-methylumbelliferyl; 4-Methylumbelliferyl-α-D-N-acetylglucosaminide; Fluorogenic α-GlcNAcase substrate
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl-2-Acetamido-2-Deoxy-α-D-Glucopyranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research and diagnostic applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) with detailed specifications are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after use to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Specific Rotation	Contact for details
Fluorogenic Activity	Functional in standard assay systems

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.