Description

H-Cys(Bzl)-Amc CAS NO 80173-27-9 is a protected cysteine derivative featuring a benzyl (Bzl) group on the sulfur atom and an aminomethylcoumarin (Amc) fluorophore. This compound serves as a critical fluorogenic substrate for sensitive protease activity assays, particularly for cysteine proteases like cathepsins. It is essential for researchers and developers in pharmaceutical discovery, biochemistry, and diagnostic kit manufacturing who require precise, high-sensitivity tools for enzyme kinetics and inhibitor screening.

Application

• Fluorogenic Protease Substrate: Primary use as a sensitive substrate for monitoring the activity of cysteine proteases (e.g., cathepsin B, L, S) in real-time kinetic assays.
• Drug Discovery & High-Throughput Screening (HTS): Enables the identification and characterization of novel protease inhibitors for therapeutic development in oncology, inflammation, and infectious diseases.
• Biochemical Research: Used in enzymology studies to determine kinetic parameters (Km, Vmax, kcat) and elucidate protease function and mechanism.
• Diagnostic Assay Development: A key component in developing fluorometric diagnostic kits for detecting specific protease activities associated with disease states.
• Apoptosis & Cell Signaling Studies: Applied in cellular assays to investigate the role of specific proteases in programmed cell death and signal transduction pathways.
• Quality Control in Biologics: Potentially used in monitoring protease activity during the production and purification of biopharmaceuticals.

Basic Information

Product Name	H-Cys(Bzl)-Amc
CAS No.	80173-27-9
Molecular Formula	C₂₀H₂₀N₂O₃S
Molecular Weight	368.45 g/mol
Synonyms	N-CBZ-L-Cysteine 7-Amido-4-Methylcoumarin; Z-Cys-AMC; CBZ-Cys-AMC; Benzyloxycarbonyl-Cys-AMC; S-Benzyl-N-[(benzyloxy)carbonyl]-L-cysteine 7-amido-4-methylcoumarin amide; L-Cysteine, N-[(benzyloxy)carbonyl]-S-(phenylmethyl)-, 2-(7-amino-4-methyl-2-oxo-2H-1-benzopyran-3-yl)ethyl ester; Fluorogenic substrate for cathepsin B/L; Cathepsin substrate Z-Cys-AMC
EINECS	Contact for details

Quality Control

Our H-Cys(Bzl)-Amc is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. A Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Assay	≥ 98.0% (on dried basis)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.