Description

L-Leucine-1-13C-15N is a stable isotope-labeled analog of the essential branched-chain amino acid L-Leucine, specifically enriched with carbon-13 at the 1-position and nitrogen-15. This compound is a critical tool for advanced research in metabolism, proteomics, and pharmaceutical development, providing precise molecular tracers for mass spectrometry and NMR studies. It is primarily utilized by research institutions, pharmaceutical companies, and diagnostic manufacturers engaged in metabolic pathway analysis, protein turnover studies, and the development of labeled internal standards.

Application

• Biomedical Research Tracer: Used in in vivo and in vitro metabolic flux analysis to study protein synthesis, degradation, and amino acid metabolism.
• Mass Spectrometry Internal Standard: Serves as a critical quantitative internal standard in LC-MS/MS and GC-MS for accurate biomarker quantification in clinical and preclinical studies.
• NMR Spectroscopy: Provides a non-radioactive, stable isotopic label for structural and dynamic studies of proteins and metabolic pathways using Nuclear Magnetic Resonance.
• Pharmaceutical Development: Employed in the research and development of new drugs, particularly in pharmacokinetic (PK) and absorption, distribution, metabolism, and excretion (ADME) studies.
• Nutritional Science Research: Used to investigate leucine metabolism, muscle protein synthesis, and the metabolic effects of dietary supplements.
• Diagnostic Assay Development: Integral to creating calibrated assays for clinical diagnostics that measure specific amino acids or related metabolites.

Basic Information

Product Name	L-Leucine-1-13C-15N
CAS No.	80134-83-4
Molecular Formula	C513CH1315NO2
Molecular Weight	135.15 g/mol
Synonyms	(2S)-2-Amino-4-methyl-1-13C-15N-pentanoic acid; L-(1-13C, 15N)Leucine; [1-13C, 15N]-L-Leucine; Isotopically labeled L-Leucine; [13C,15N]-Leu; 13C,15N-L-Leucine; (S)-2-Amino-4-methyl[1-13C,15N]valeric acid
EINECS	Contact for details

Quality Control

Our L-Leucine-1-13C-15N is manufactured under strict quality control protocols to ensure the highest isotopic and chemical purity for sensitive research applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (Atom % 13C and 15N), chemical purity, and chiral purity. We adhere to relevant standards for research-grade biochemicals, ensuring reliability and consistency for your critical experiments.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Isotopic Enrichment (Atom % 13C)	≥ 99%
Isotopic Enrichment (Atom % 15N)	≥ 98%
Optical Rotation	[α]D20: +14.5° to +16.5° (c=2 in 6N HCl)
Chiral Purity (HPLC)	≥ 99.0% L-isomer
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.