Description

Abz-Gly-Ala-Lys(Ac)-Ala-Ala-Dap(Dnp)-Nh2 is a specialized, sequence-defined synthetic peptide containing a 2,4-dinitrophenyl (Dnp) quencher group and an ortho-aminobenzoic acid (Abz) fluorophore, making it a critical tool for fluorescence resonance energy transfer (FRET) studies. This compound is primarily designed for use as a highly specific and sensitive substrate for proteolytic enzymes, particularly in kinetic assays and inhibitor screening. It is essential for researchers and developers in pharmaceutical R&D, biochemical research, and diagnostic kit manufacturing who require precise tools for studying enzyme mechanisms and activity.

Application

• Protease/Enzyme Substrate: Acts as a FRET-based quenched fluorescent substrate for kinetic analysis of serine proteases, including elastase, cathepsin G, and related enzymes.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize novel enzyme inhibitors for therapeutic development.
• Biochemical Mechanism Studies: Enables detailed investigation of enzyme specificity, catalytic efficiency, and cleavage sites.
• Diagnostic Assay Development: Serves as a key component in the development of sensitive fluorometric assays for clinical and research diagnostics.
• Quality Control in Biologics: Employed to monitor and quantify protease activity during the production and purification of biopharmaceuticals.
• Academic Research: A vital reagent in university and institutional labs for fundamental research in biochemistry, cell biology, and enzymology.

Basic Information

Product Name	Abz-Gly-Ala-Lys(Ac)-Ala-Ala-Dap(Dnp)-Nh2
CAS No.	761443-02-1
Molecular Formula	C46H60N14O17
Molecular Weight	1081.06 g/mol
Synonyms	Abz-Gly-Ala-Lys(Ac)-Ala-Ala-Dap(Dnp)-Amide; FRET Substrate for Human Neutrophil Elastase; Abz-Gly-Ala-Lys(Ac)-Ala-Ala-Dap(Dnp)-NH2; HNE Fluorogenic Substrate; o-Aminobenzoic Acid-Gly-Ala-Lys(Ac)-Ala-Ala-2,3-Diaminopropionic Acid(Dnp)-Amide; Peptide Substrate 761443-02-1; Abz-Gly-Ala-Lys(Ac)-Ala-Ala-Dap(2,4-DNP)-NH2
EINECS	Contact for details

Quality Control

Our Abz-Gly-Ala-Lys(Ac)-Ala-Ala-Dap(Dnp)-Nh2 is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification, mass spectrometry (MS) for identity confirmation, and amino acid analysis (AAA). Certificates of Analysis (COA) detailing all specifications are provided and available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Peptide Content	≥ 70%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.