Description

Maleimide-PEG4-NHS Ester is a heterobifunctional crosslinker featuring a maleimide group for thiol conjugation and an NHS ester for primary amine coupling. This compound is critical for creating stable, site-specific bioconjugates with controlled spacing due to its hydrophilic PEG4 spacer. It is essential for researchers and manufacturers in biopharmaceutical development, diagnostic assay production, and advanced materials science who require precise and reliable protein labeling, antibody-drug conjugate (ADC) synthesis, and surface functionalization.

Application

• Antibody-Drug Conjugate (ADC) Linker: Site-specific conjugation of cytotoxic payloads to cysteine residues on monoclonal antibodies.
• Protein and Peptide Labeling: Fluorescent or affinity tag attachment to proteins via free thiol or amine groups for imaging and detection.
• Surface Functionalization: Immobilization of biomolecules (e.g., peptides, antibodies) onto biosensor chips, nanoparticles, or other solid supports.
• PEGylation Reagent: Introduction of a polyethylene glycol (PEG) chain to biomolecules to enhance solubility, stability, and pharmacokinetic properties.
• Diagnostic Reagent Synthesis: Production of enzyme conjugates and labeled antigens/antibodies for ELISA and lateral flow assays.
• Hydrogel and Biomaterial Fabrication: Crosslinking agent for creating biocompatible polymer networks with tailored properties.

Basic Information

Product Name	Maleimide-PEG4-NHS Ester
CAS No.	756525-99-2
Molecular Formula	C23H34N2O11
Molecular Weight	514.53 g/mol
Synonyms	N-(2-{2-[2-(2-{3-[(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)oxy]-3-oxopropoxy}ethoxy)ethoxy]ethoxy}ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide; Maleimide-PEG4-NHS; NHS-PEG4-Maleimide; 4-Arm PEG-Maleimide (NHS ester derivative); SM(PEG)4; MAL-PEG4-NHS; PEG4 crosslinker, NHS-Maleimide
EINECS	Contact for details

Quality Control

Our Maleimide-PEG4-NHS Ester is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry to ensure identity and consistency. Certificates of Analysis (COA) with detailed specifications are provided for every shipment to support your research, development, and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.