Description

Ubiquitin CAS NO 79586-22-4 is a highly conserved regulatory protein found in all eukaryotic organisms, playing a fundamental role in the ubiquitin-proteasome system for targeted protein degradation. This small protein is essential for critical cellular processes including cell cycle regulation, DNA repair, and immune response signaling. It is a vital reagent for researchers and manufacturers in the fields of proteomics, biochemistry, and pharmaceutical development, particularly for studying protein turnover, signaling pathways, and developing novel therapeutic targets.

Application

• Biochemical Research: A fundamental reagent for in vitro ubiquitination assays, studying enzyme kinetics of E1, E2, and E3 ligases, and investigating the mechanisms of the 26S proteasome.
• Drug Discovery & Development: Used in high-throughput screening (HTS) platforms to identify and validate novel inhibitors of the ubiquitin-proteasome pathway for oncology, neurodegenerative, and inflammatory diseases.
• Proteomics & Protein Interaction Studies: Essential for pull-down assays, mass spectrometry analysis, and mapping protein-protein interaction networks dependent on ubiquitin signaling.
• Antibody & Diagnostic Kit Production: Serves as a key antigen for generating highly specific monoclonal and polyclonal antibodies used in Western blotting, ELISA, and immunohistochemistry.
• Enzyme Substrate: Acts as a substrate for deubiquitinating enzymes (DUBs) in activity assays and inhibitor screening for therapeutic development.
• Cell Signaling Studies: Utilized to investigate the role of ubiquitination in NF-κB activation, receptor endocytosis, and other critical signal transduction pathways.

Basic Information

Product Name	Ubiquitin
CAS No.	79586-22-4
Molecular Formula	C378H630N105O118S
Molecular Weight	8564.9 g/mol
Synonyms	Ubiquitin (UBQ); Ubiquitin Protein; Polyubiquitin; Ubiquitin (Human); RPS27A; UBB; UBC; UBA52; Ubiquitin-40S ribosomal protein S27a; Ubiquitin-60S ribosomal protein L40; Ubiquitin and ribosomal protein S27a; Glyceraldehyde-3-phosphate dehydrogenase
EINECS	Contact for details

Quality Control

Our Ubiquitin is produced and tested under stringent conditions to ensure the highest standards of purity and biological activity, suitable for sensitive research and development applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional activity. We adhere to rigorous quality management protocols to guarantee batch-to-batch consistency and reliability for your critical workflows.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/MS)	Conforms to standard
Purity (HPLC)	≥ 95%
Protein Content (UV)	≥ 90%
Biological Activity (Ubiquitination Assay)	Active
Endotoxin Level (LAL)	< 1.0 EU/μg
Solubility	Soluble in water or buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.