Description

4-Hydroxymephobarbital is a key pharmaceutical intermediate and reference standard, primarily recognized as a major metabolite of the sedative-hypnotic drug mephobarbital. Its precise chemical structure and high purity are critical for analytical and research applications, ensuring accurate metabolic profiling and pharmacokinetic studies. This compound is essential for pharmaceutical R&D laboratories, forensic toxicology, and manufacturers of reference materials who require reliable, well-characterized substances for method development and quality control.

Application

• Pharmaceutical Reference Standard: Used as a certified reference material (CRM) for the quantitative and qualitative analysis of mephobarbital and its metabolites in drug testing and bioanalytical studies.
• Metabolite Research: Serves as a critical standard in pharmacokinetic and metabolic pathway studies for barbiturate-class pharmaceuticals.
• Forensic Toxicology: Employed in the development and validation of analytical methods (e.g., GC-MS, LC-MS/MS) for the detection of barbiturates in biological samples.
• Impurity Profiling: Used in the quality control of active pharmaceutical ingredients (APIs) to identify and quantify related substances and degradation products.
• Academic & Contract Research: Utilized in university and CRO laboratories for pharmacological research and the study of cytochrome P450 enzyme activity related to drug metabolism.

Basic Information

Product Name	4-Hydroxymephobarbital
CAS No.	79025-08-4
Molecular Formula	C13H14N2O4
Molecular Weight	262.26 g/mol
Synonyms	5-Ethyl-1-methyl-5-(4-hydroxyphenyl)barbituric Acid; 4'-Hydroxymephobarbital; Mephobarbital 4'-Hydroxy Metabolite; 5-(4-Hydroxyphenyl)-5-ethyl-1-methylpyrimidine-2,4,6(1H,3H,5H)-trione; 1-Methyl-5-ethyl-5-(4-hydroxyphenyl)barbituric Acid; N-Methylphenobarbital 4'-Hydroxy Derivative; Ro 05-4200/4
EINECS	Contact for details

Quality Control

Our 4-Hydroxymephobarbital is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including identification by spectroscopic methods (IR, NMR), purity assay by HPLC, and control of related substances and residual solvents. A Certificate of Analysis (COA) detailing all test results and specifications is provided with every shipment to ensure full traceability and compliance with your research or quality standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The container should be kept in a well-ventilated area and protected from moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time corresponds to reference standard
Assay (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0% Any single impurity ≤ 1.0%
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.