Description

Dispase CAS NO 78990-62-2 is a neutral, microbial metalloprotease enzyme known for its specific activity in cleaving fibronectin and collagen IV, facilitating the gentle dissociation of cells from tissues and substrates. This product matters for its high purity and consistent activity, which are critical for reproducible results in sensitive biological applications. It is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and cell therapy industries who require a reliable, non-mammalian enzyme for primary cell culture, tissue engineering, and organoid research.

Application

• Primary Cell Isolation and Culture: Gently dissociates epithelial cells, hepatocytes, and other cell types from tissues while maintaining high viability and functionality.
• Stem Cell Research: Used for the passaging and harvesting of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) without damaging cell surface markers.
• Organoid Generation and Maintenance: Facilitates the dissociation of tissue fragments for organoid initiation and the subsequent splitting of mature organoids.
• Tissue Engineering: Aids in the preparation of acellular tissue matrices by removing cells while preserving the underlying extracellular matrix structure.
• Cancer Research: Employed in the isolation of primary tumor cells for the establishment of patient-derived xenograft (PDX) models and in vitro studies.
• Mammary Gland Biology: Specifically useful for the isolation of mammary epithelial cells and the study of mammary gland morphogenesis.
• Keratinocyte Culture: The enzyme of choice for detaching keratinocytes from culture flasks without using trypsin, which can damage cell surface receptors.
• Biopharmaceutical Production: Applied in cell-based vaccine and therapeutic protein manufacturing processes where gentle cell harvesting is required.

Basic Information

Product Name	Dispase
CAS No.	78990-62-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Dispase I; Dispase Neutral Protease; Bacillus polymyxa Neutral Protease; Microbial Neutral Metalloprotease; EC 3.4.24.4; Dispase II (Note: Often used interchangeably, though Dispase II is a different enzyme); Thermolysin-like Neutral Protease
EINECS	Contact for details

Quality Control

Our Dispase is manufactured under strict quality management systems. Each batch undergoes rigorous testing for enzymatic activity, purity (via SDS-PAGE and HPLC), and the absence of contaminating proteases (e.g., trypsin, chymotrypsin) and endotoxins. We provide Certificates of Analysis (COA) detailing specific activity (units/mg), protein content, and sterility data (where applicable) to ensure it meets the stringent requirements for research and cGMP manufacturing.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. The product is hygroscopic (moisture-sensitive). For long-term stability, consider storage at -20°C. Allow the vial to equilibrate to room temperature before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (IR)	Conforms
Specific Activity	> 5.0 units/mg protein
Protein Content (Biuret)	Contact for details
Purity (SDS-PAGE)	> 90% single band
Endotoxin (LAL)	< 0.1 EU/μg (for cell culture grade)
Sterility (Membrane Filtration)	Sterile (for sterile filtered grade)
Solubility	Soluble in water or buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.