Description

Poly-D-Lysine is a synthetic, positively charged polymer of the D-isomer of the amino acid lysine, widely recognized for its cell culture applications. Its key value lies in its ability to coat surfaces, promoting the adhesion of a wide variety of cells, including neurons and other anchorage-dependent cell types. This product is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and life sciences industries who require reliable substrates for cell-based assays, tissue engineering, and diagnostic development.

Application

• Cell Culture Substrate Coating: Used to coat glass, plastic, and other surfaces to enhance the attachment and growth of adherent cells in vitro.
• Neuroscience Research: Particularly valuable for promoting the adhesion of primary neurons and other sensitive cell types in neurobiological studies.
• Biochip and Microarray Fabrication: Serves as a functional layer to immobilize biomolecules or cells on biosensor and diagnostic chip surfaces.
• Tissue Engineering: Applied as a coating on scaffolds to improve cell seeding efficiency and initial cell attachment.
• In Vitro Diagnostics (IVD): Used in the preparation of slides and surfaces for cell-based diagnostic tests.
• Virology and Microbiology: Facilitates the adhesion of certain cell lines used in viral plaque assays and other microbiological applications.

Basic Information

Product Name	Poly-D-Lysine
CAS No.	26853-89-4
Molecular Formula	(C6H12N2O)n • xHBr
Molecular Weight	Varies (Polymer)
Synonyms	PDL; Poly(D-lysine hydrobromide); D-Lysine homopolymer; Poly-D-lysine HBr; P(D-Lys); (2R)-2,6-Diaminohexanoic acid homopolymer; NSC 106982; Poly(imino[(2R)-2-amino-1-carboxy-1,5-pentanediyl])
EINECS	Contact for details

Quality Control

Our Poly-D-Lysine is produced under controlled conditions to ensure batch-to-batch consistency suitable for research and industrial applications. Quality is verified through analytical methods including spectroscopy and chromatography. While specific grades may vary, we ensure compliance with relevant quality benchmarks. A Certificate of Analysis (COA) detailing purity, molecular weight range, and other critical parameters is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at room temperature (15-25°C) in a dry environment. For long-term stability, desiccate if the container is opened frequently. Keep away from strong oxidizing agents.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (IR)	Conforms
Assay (Poly-D-Lysine base)	≥ 95.0% (by HPLC or Ninhydrin)
Molecular Weight Range	30,000 - 70,000 Da (Typical, other ranges available)
Solubility (in Water)	Clear, colorless solution at 1 mg/mL
pH (1% solution)	6.0 - 8.0
Endotoxin	< 1.0 EU/mg (for cell culture grades)
Bioprocessing Performance	Supports cell adhesion as tested

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.