Description

Lb Broth CAS NO 12111-19-2 is a complex, non-selective microbiological growth medium widely used for the cultivation of a broad spectrum of bacteria, particularly Escherichia coli. Its standardized formulation ensures consistent, high-yield cell growth, which is critical for reliable experimental and industrial-scale results. This product is essential for researchers and manufacturers in biotechnology, molecular biology, and pharmaceutical development who require robust bacterial cultures for cloning, protein expression, and fermentation processes.

Application

• Molecular Biology & Cloning: Standard medium for growing recombinant E. coli strains during plasmid propagation and DNA extraction.
• Protein Expression: Supports high-density bacterial fermentation for the production of recombinant proteins and enzymes.
• Microbiological Research: A general-purpose broth for the isolation, cultivation, and maintenance of non-fastidious bacterial cultures.
• Quality Control (QC) Testing: Used in pharmaceutical and food industry QC labs for microbial limit tests and sterility validation studies.
• Antibiotic Susceptibility Testing: Serves as a base medium for preparing inoculums in standardized antimicrobial susceptibility assays.
• Seed Culture Preparation: Ideal for generating active, healthy starter cultures for scale-up in industrial bioprocesses.

Basic Information

Product Name	Lb Broth
CAS No.	12111-19-2
Molecular Formula	Complex Mixture
Molecular Weight	N/A (Mixture)
Synonyms	Luria-Bertani Broth; LB Broth; LB Medium; Luria Broth; Lysogeny Broth; Miller's LB Broth; Lennox L Broth; Bacterial Culture Broth; Tryptone-Yeast Extract-NaCl Medium
EINECS	Contact for details

Quality Control

Our Lb Broth is manufactured under strict quality management systems to ensure batch-to-batch consistency and performance. Each lot undergoes rigorous microbiological and physicochemical testing to meet the demanding requirements of research and industrial applications. A Certificate of Analysis (COA) detailing specifications such as pH, clarity, gel strength (for agar versions), growth promotion, and sterility (where applicable) is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent clumping and degradation due to atmospheric moisture.

Specification

Item	Specification
Appearance	Homogeneous free-flowing powder
Color	Light beige to yellow
Solubility (1% in Water)	Clear to slightly opalescent solution
pH (1% Solution, 25°C)	7.0 ± 0.2
Gelling Property (Agar Form)	Firm gel at 1.5% (w/v)
Growth Promotion	Supports typical growth of E. coli ATCC 25922
Endotoxin (if specified)	< 0.25 EU/mL (for cell culture grade)
Heavy Metals (as Pb)	< 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.